김한솔 (University of California San Diego) | 한빛사논문 > 한빛사

한빛사논문

조회442

김한솔Han Sol Kim

University of California San Diego

Nat. Commun., Apr 30 2024, 15 (1) | https://doi.org/10.1038/s41467-024-47132-8 Biocomposite thermoplastic polyurethanes containing evolved bacterial spores as living fillers to facilitate polymer disintegration

Han Sol Kim^1,8, Myung Hyun Noh^2,3,8, Evan M. White ⁴, Michael V. Kandefer ⁴, Austin F. Wright ⁴, Debika Datta ¹, Hyun Gyu Lim², Ethan Smiggs ², Jason J. Locklin ⁴, Md Arifur Rahman ⁵, Adam M. Feist^2,6 & Jonathan K. Pokorski ^1,7

¹Department of NanoEngineering, University of California San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA.

²Department of Bioengineering, University of California San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA.

³Research Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology(KRICT), 406-30 Jongga-ro, Ulsan 44429, Republic of Korea.

⁴New Materials Institute, University of Georgia, Athens, GA 30602, USA.

⁵Thermoplastic Polyurethane Research, BASF Corporation, 1609 Biddle Ave., Wyandotte, MI 48192, USA.

⁶The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet, 2800 Kgs, Lyngby, Denmark.

⁷Institute for Materials Discovery and Design, University of California San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA.

⁸These authors contributed equally: Han Sol Kim, Myung Hyun Noh.

Correspondence to Md Arifur Rahman, Adam M. Feist or Jonathan K. Pokorski.

Abstract

The field of hybrid engineered living materials seeks to pair living organisms with synthetic materials to generate biocomposite materials with augmented function since living systems can provide highly-programmable and complex behavior. Engineered living materials have typically been fabricated using techniques in benign aqueous environments, limiting their application. In this work, biocomposite fabrication is demonstrated in which spores from polymer-degrading bacteria are incorporated into a thermoplastic polyurethane using high-temperature melt extrusion. Bacteria are engineered using adaptive laboratory evolution to improve their heat tolerance to ensure nearly complete cell survivability during manufacturing at 135 °C. Furthermore, the overall tensile properties of spore-filled thermoplastic polyurethanes are substantially improved, resulting in a significant improvement in toughness. The biocomposites facilitate disintegration in compost in the absence of a microbe-rich environment. Finally, embedded spores demonstrate a rationally programmed function, expressing green fluorescent protein. This research provides a scalable method to fabricate advanced biocomposite materials in industrially-compatible processes.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-05-03)
연구진국내+국외 연구진
분야 생명과학 > 생물공학

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