한빛사논문
- 조회318
Joonwon Kim 1 2, Alexander F Kratz 1, Shiye Chen 2, Jenny Sheng 1, Hark Kyun Kim 2, Liudeng Zhang 1 2 3, Brijesh Kumar Singh 1, Alejandro Chavez 1 2 *
1Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY 10032, USA.
2Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA.
3College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
*Corresponding author: correspondence to Alejandro Chavez
Abstract
To facilitate the interrogation of protein function at scale, we have developed high-throughput insertion of tags across the genome (HITAG). HITAG enables users to rapidly produce libraries of cells, each with a different protein of interest C-terminally tagged. HITAG is based on a modified strategy for performing Cas9-based targeted insertions, coupled with an improved approach for selecting properly tagged lines. Analysis of the resulting clones generated by HITAG reveals high tagging specificity, with most successful tagging events being indel free. Using HITAG, we fuse mCherry to a set of 167 stress granule–associated proteins and elucidate the features that drive a subset of proteins to strongly accumulate within these transient RNA-protein granules.
논문정보
- Research article
- 2024년 05월 (BRIC 등록일 2024-05-27)
- 국외 연구진
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