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민준원Junwon Min

서강대학교

Adv. Mater., Apr 25 2024, | https://doi.org/10.1002/adma.202403896 Capillarity-driven Enrichment and Hydrodynamic Trapping of Trace Nucleic Acids by Plasmonic Cavity Membrane for Rapid and Sensitive Detections

Keumrai Whang ^1,2, Junwon Min ³, Yonghee Shin ^1,2, Inhyeok Hwang ^1,2, Hyunjoo Lee ³, Taejin Kwak ³, Ju A La ^1,2, Sungbong Kim ^2,4, Dongchoul Kim ³, Luke P. Lee ^5,6,7, Taewook Kang^1,2

¹Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 04107, South Korea.

²Institute of Integrated Biotechnology, Sogang University, Seoul, 04107, South Korea.

³Department of Mechanical Engineering, Sogang University, Seoul, 04107, South Korea.

⁴Department of Chemistry, Military Academy, Seoul, 01805, South Korea.

⁵Harvard Institute of Medicine, Harvard Medical School, Harvard University, Brigham and Women's Hospital, Boston, MA, 02115, USA.

⁶Department of Bioengineering, Department of Electrical Engineering and Computer Science, University of California at Berkeley, Berkeley, CA, USA.

⁷Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwonsi, Gyeonggi-do, 16419, South Korea.

K. Whang, J. Min, and Y. Shin contributed equally to this work.

CORRESPONDING AUTHORS : Dongchoul Kim, Luke P. Lee, Taewook Kang

Abstract

Small reactor-based Polymerase chain reaction (PCR) has attracted considerable attention. A significant number of tiny reactors must be prepared in parallel to capture, amplify and accurately quantify few target genes in clinically relevant large volume, which, however, requires sophisticated microfabrication and longer sample-to-answer time. Here we report single plasmonic cavity membrane that not only enriches and captures few nucleic acids by taking advantage of both capillarity and hydrodynamic trapping but also quickly amplifies them for sensitive plasmonic detection. We fabricate the plasmonic cavity membrane with few nanoliters in a void volume by self-assembling gold nanorods with SiO ₂ tips. Simulations reveal that hydrodynamic stagnation between the SiO ₂ tips is mainly responsible for the trapping of the nucleic acid in the membrane. Finally, we show that our plasmonic cavity membrane is capable of enriching SARS-CoV-2 genes up to 20000-fold within 1 min, amplifying within 3 min, and detecting the trace genes as low as a single copy per microliter. We anticipate that our work not only expands the utility of PCR but also provide an innovative way of the enrichment and detection of trace biomolecules in a variety of point-of-care testing applications. This article is protected by copyright. All rights reserved.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-29)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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