김동명 (충남대학교)

한빛사논문

조회217

충남대학교

CV File 306 kb

Biosens. Bioelectron., Apr 23 2024, 257 S0956-5663(24)00336-1 | https://doi.org/10.1016/j.bios.2024.116331 A cell-free biosensor for multiplexed and sensitive detection of biological warfare agents

Yu Jin Park ^a, Sunjoo Choi ^b, Kyung Won Lee ^b, So-Yoon Park ^a, Dong-Yeon Song^a, Tae Hyeon Yoo ^b, Dong-Myung Kim ^a

^aDepartment of Chemical Engineering and Applied Chemistry, Chungnam National University, 99 Daehak-ro, Daejeon, 34134, Republic of Korea

^bDepartment of Molecular Science and Technology, Ajou University, 206 World cup-ro, Suwon, 16499, Republic of Korea

Corresponding authors: Tae Hyeon Yoo, Dong-Myung Kim

Abstract

The rapid and precise detection of pathogenic agents is critical for public health and societal stability. The detection of biological warfare agents (BWAs) is especially vital within military and counter-terrorism contexts, essential in defending against biological threats. Traditional methods, such as polymerase chain reaction (PCR), are limited by their need for specific settings, impacting their adaptability and versatility. This study introduces a cell-free biosensor for BWA detection by converting the 16S rRNA of targeted pathogens into detectable functional protein molecules. The modular nature of this approach allows for the flexible configuration of pathogen detection, enabling the simultaneous identification of multiple pathogenic 16S rRNAs through customized reporter proteins for each targeted sequence. Furthermore, we demonstrate how this method integrates with techniques utilizing retroreflective Janus particles (RJPs) for facile and highly sensitive pathogen detection. The cell-free biosensor, employing RJPs to measure the reflection of non-chromatic white light, can detect 16S rRNA from BWAs at femtomolar levels, corresponding to tens of colony-forming units per milliliter of pathogenic bacteria. These findings represent a significant advancement in pathogen detection, offering a more efficient and accessible alternative to conventional methodologies.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-29)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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