신성철 (Stanford University, 서울대학교)

한빛사논문

조회309

Stanford University, 서울대학교

CV File 199 kb

Adv. Funct. Mater., Dec 08 2023, 33 (50) 2307435 | https://doi.org/10.1002/adfm.202307435 Gelation of Uniform Interfacial Diffusant in Embedded 3D Printing

Sungchul Shin ^1,2, Lucia G. Brunel ³, Betty Cai ¹, David Kilian ¹, Julien G. Roth ⁴, Alexis J. Seymour ⁵, Sarah C. Heilshorn¹

¹Department of Materials Science and Engineering, Stanford University, 466 Lomita Mall, Stanford, CA 94305, USA.

²Department of Agriculture, Forestry, and Bioresources, Seoul National University, 08826 Gwanak-ro 1, Gwanak-gu, Seoul, Republic of Korea.

³Department of Chemical Engineering, Stanford University, 466 Lomita Mall, Stanford, CA 94305, USA.

⁴Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 466 Lomita Mall, Stanford, CA 94305, USA.

⁵Department of Bioengineering, Stanford University, 466 Lomita Mall, Stanford, CA 94305, USA.

CORRESPONDING AUTHORS: Sungchul Shin, Sarah C. Heilshorn

Abstract

While the human body has many different examples of perfusable structures with complex geometries, biofabrication methods to replicate this complexity are still lacking. Specifically, the fabrication of self-supporting, branched networks with multiple channel diameters is particularly challenging. Here, we present the Gelation of Uniform Interfacial Diffusant in Embedded 3D Printing (GUIDE-3DP) approach for constructing perfusable networks of interconnected channels with precise control over branching geometries and vessel sizes. To achieve user-specified channel dimensions, this technique leverages the predictable diffusion of crosslinking reaction-initiators released from sacrificial inks printed within a hydrogel precursor. We demonstrate the versatility of GUIDE-3DP to be adapted for use with diverse physicochemical crosslinking mechanisms by designing seven printable material systems. Importantly, GUIDE-3DP allows for the independent tunability of both the inner and outer diameters of the printed channels and the ability to fabricate seamless junctions at branch points. This 3D bioprinting platform is uniquely suited for fabricating lumenized structures with complex shapes characteristic of multiple hollow vessels throughout the body. As an exemplary application, we demonstrate the fabrication of vasculature-like networks lined with endothelial cells. GUIDE-3DP represents an important advance toward the fabrication of self-supporting, physiologically relevant networks with intricate and perfusable geometries.

논문정보

형식Research article
게재일2023년 12월 (BRIC 등록일 2024-04-23)
연구진국내(교신)+국외 연구진
분야 생명과학 > 농림생태환경

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