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Probir Kumar MittraProbir Kumar Mittra

충북대학교

J. Hazard. Mater., Apr 09 2024, 471 S0304-3894(24)00841-0 | https://doi.org/10.1016/j.jhazmat.2024.134262 Deciphering proteomic mechanisms explaining the role of glutathione as an aid in improving plant fitness and tolerance against cadmium-toxicity in Brassica napus L

Probir Kumar Mittra ^a, Md Atikur Rahman ^b, Swapan Kumar Roy ^c, Soo-Jeong Kwon ^a, Sung Ho Yun ^d, Cho Kun ^d, Meiliang Zhou^e, Tomoyuki Katsube-Tanaka^f, Tatsuhiko Shiraiwa ^f, Sun-Hee Woo ^a

^aDepartment of Crop Science, Chungbuk National University, Cheong-ju 28644, Republic of Korea

^bGrassland and Forage Division, Rural Development Administration, National Institute of Animal Science, Cheonan 31000, Republic of Korea

^cCollege of Agricultural Sciences, IUBAT—International University of Business Agriculture and Technology, 4 Embankment Drive Road, Sector 10 Uttara Model Town, Dhaka 1230, Bangladesh

^dBio-Chemical Analysis Team, Center for Research Equipment, Korea Basic Science Institute, Ochang, Cheong-ju 28119, Republic of Korea

^eInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, 12 South Zhongguancun Street, Haidian, Beijing 100081, China

^fGraduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606–8502, Japan

Corresponding author : Sun-Hee Woo

Abstract

Cadmium (Cd) hazard is a serious limitation to plants, soils and environments. Cd-toxicity causes stunted growth, chlorosis, necrosis, and plant yield loss. Thus, ecofriendly strategies with understanding of molecular mechanisms of Cd-tolerance in plants is highly demandable. The Cd-toxicity caused plant growth retardation, leaf chlorosis and cellular damages, where the glutathione (GSH) enhanced plant fitness and Cd-toxicity in Brassica through Cd accumulation and antioxidant defense. A high-throughput proteome approach screened 4947 proteins, wherein 370 were differently abundant, 164 were upregulated and 206 were downregulated. These proteins involved in energy and carbohydrate metabolism, CO ₂ assimilation and photosynthesis, signal transduction and protein metabolism, antioxidant defense response, heavy metal detoxification, cytoskeleton and cell wall structure, and plant development in Brassica. Interestingly, several key proteins including glutathione S-transferase F9 (A0A078GBY1), ATP sulfurylase 2 (A0A078GW82), cystine lyase CORI3 (A0A078FC13), ferredoxin-dependent glutamate synthase 1 (A0A078HXC0), glutaredoxin-C5 (A0A078ILU9), glutaredoxin-C2 (A0A078HHH4) actively involved in antioxidant defense and sulfur assimilation-mediated Cd detoxification process confirmed by their interactome analyses. These candidate proteins shared common gene networks associated with plant fitness, Cd-detoxification and tolerance in Brassica. The proteome insights may encourage breeders for enhancing multi-omics assisted Cd-tolerance in Brassica, and GSH-mediated hazard free oil seed crop production for global food security.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-25)
연구진국내(교신)+국외 연구진
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