한빛사
>
한빛사논문
한빛사논문
- 조회209
J. Med. Virol., Apr 2024, 96 (4) | https://doi.org/10.1002/jmv.29605
Antiviral activity of adenoviral vector expressing human interferon lambda-4 against influenza virus
Dong-Hwi Kim 1, Jae-Hyeong Kim 1, Kyu-Beom Lim 1, Joong-Bok Lee 1,2, Seung-Yong Park 1,2, Chang-Seon Song 1,2, Sang-Won Lee 1,2, Dong-Hun Lee 1,2, In-Soo Choi 1,2,3
1Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
2Konkuk University Zoonotic Diseases Research Center, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
3KU Center for Animal Blood Medical Science, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
CORRESPONDING AUTHOR : In-Soo Choi
Abstract
Interferon lambda (IFNλ), classified as a type III IFN, is a representative cytokine that plays an important role in innate immunity along with type I IFN. IFNλ can elicit antiviral states by inducing peculiar sets of IFN-stimulated genes (ISGs). In this study, an adenoviral vector expression system with a tetracycline operator system was used to express human IFNλ4 in cells and mice. The formation of recombinant adenovirus (rAd-huIFNλ4) was confirmed using immunohistochemistry assays and transmission electron microscopy. Its purity was verified by quantifying host cell DNA and host cell proteins, as well as by confirming the absence of the replication-competent adenovirus. The transduction of rAd-huIFNλ4 induced ISGs and inhibited four subtypes of the influenza virus in both mouse-derived (LA-4) and human-derived cells (A549). The antiviral state was confirmed in BALB/c mice following intranasal inoculation with 10
9 PFU of rAd-huIFNλ4, which led to the inhibition of four subtypes of the influenza virus in mouse lungs, with reduced inflammatory lesions. These results imply that human IFNλ4 could induce antiviral status by modulating ISG expression in mice.
논문정보
- Research article
- 2024년 04월 (BRIC 등록일 2024-04-19)
- 국내 연구진
- 생명과학 > 유전자발현
댓글 작성 로그인
팝업 닫기관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
