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Enkhchimeg LkhagvaEnkhchimeg Lkhagva

전북대학교

Gut Microbes, Published online: 18 Apr 2024 | https://doi.org/10.1080/19490976.2024.2342497 The gut microbe pair of Oribacterium sp. GMB0313 and Ruminococcus sp. GMB0270 confers complete protection against SARS-CoV-2 infection by activating CD8+ T cell-mediated immunity

Mingda Wang^a,b*, Enkhchimeg Lkhagva^a*, Sura Kim^a*, Chongkai Zhai^a,c, Md Minarul Islama, Hyeon J. Kimd, and Seong-Tshool Honga

aDepartment of Biomedical Sciences, Jeonbuk National University Medical School, Jeollabuk-Do, South Korea;

bDepartment of Critical Care Medicine, Shandong Provincial Hospital affiliated with Shandong First Medical University, Jinan, China;

cCollege of Food and Drugs, Luoyang Polytechnic, Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, Henan Province, China;

dBioLabs-LA at the Lundquist Institute for Bio Medical Innovation at Harbor UCLA, SNJ Pharma Inc, Torrance, CA, USA

^*These authors contributed equally to this work.

CONTACT: Seong-Tshool Hong, Hyeon J. Kim

Abstract

Despite the potential protective role of the gut microbiome against COVID-19, specific microbes conferring resistance to COVID-19 have not yet been identified. In this work, we aimed to identify and validate gut microbes at the species level that provide protection against SARS-CoV-2 infection. To identify gut microbes conferring protection against COVID-19, we conducted a fecal microbiota transplantation (FMT) from an individual with no history of COVID-19 infection or immunization into a lethal COVID-19 hamster model. FMT from this COVID-19-resistant donor resulted in significant phenotypic changes related to COVID-19 sensitivity in the hamsters. Metagenomic analysis revealed distinct differences in the gut microbiome composition among the hamster groups, leading to the identification of two previously unknown bacterial species: Oribacterium sp. GMB0313 and Ruminococcus sp. GMB0270, both associated with COVID-19 resistance. Subsequently, we conducted a proof-of-concept confirmation animal experiment adhering to Koch's postulates. Oral administration of this gut microbe pair, Oribacterium sp. GMB0313 and Ruminococcus sp. GMB0270, to the hamsters provided complete protection against SARS-CoV-2 infection through the activation of CD8+ T cell mediated immunity. The prophylactic efficacy of the gut microbe pair against SARS-CoV-2 infection was comparable to, or even superior to, current mRNA vaccines. This strong prophylactic efficacy suggests that the gut microbe pair could be developed as a host-directed universal vaccine for all betacoronaviruses, including potential future emerging viruses.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-22)
연구진국내(교신)+국외 연구진
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