신아영 (한국생명공학연구원)

한빛사논문

조회550

한국생명공학연구원

CV File 178 kb

Sci. Data, Apr 16 2024, 11 (1) | https://doi.org/10.1038/s41597-024-03232-0 High-quality chromosome-level genome assembly of Nicotiana benthamiana

Seo-Rin Ko^1,2,6, Sanghee Lee^1,3,6, Hyunjin Koo^1,6, Hyojeong Seo⁴, JaewoongYu⁴, Yong-Min Kim^1,2,5, Suk-Yoon Kwon^1,3 & Ah-Young Shin^1,2

¹Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.

²Department of Bioinformatics, KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.

³Biosystems and Bioengineering Program, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), Daejeon, 34113, Korea.

⁴UNGENE, Seoul, 08592, Republic of Korea.

⁵Digital Biotech Innovation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.

⁶These authors contributed equally: Seo-Rin Ko, Sanghee Lee, Hyunjin Koo.

Corresponding authors :Correspondence to Yong-Min Kim, Suk-Yoon Kwon or Ah-Young Shin.

Abstract

Nicotiana benthamiana is a fundamental model organism in plant research. Recent advancements in genomic sequencing have revealed significant intraspecific genetic variations. This study addresses the pressing need for a precise genome sequence specific to its geographic origin by presenting a comprehensive genome assembly of the N. benthamiana LAB strain from the Republic of Korea (NbKLAB). We compare this assembly with the widely used NbLAB360 strain, shedding light on essential genomic differences between them. The outcome is a high-quality, chromosome-level genome assembly comprising 19 chromosomes, spanning 2,762 Mb, with an N50 of 142.6 Mb. Comparative analyses revealed notable variations, including 46,215 protein-coding genes, with an impressive 99.5% BUSCO completeness score. Furthermore, the NbKLAB assembly substantially improved the QV from 33% for NbLAB360 to 49%. This refined chromosomal genome assembly for N. benthamiana, in conjunction with comparative insights, provides a valuable resource for genomics research and molecular biology. This accomplishment forms a strong foundation for in-depth exploration into the intricacies of plant genetics and genomics, improved precision, and a comparative framework.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-18)
연구진국내 연구진
분야 생명과학 > 생화학

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