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J. Am. Soc. Nephrol., Apr 15 2024, | https://doi.org/10.1681/ASN.0000000000000354 Cell Type- and Age-Specific Expression of lncRNAs across Kidney Cell Types

Kim, Gyeong Dae^1,*; Shin, So-I^1,*; Jung, Su Woong^1,2; An, Hyunsu¹; Choi, Sin Young¹; Eun, Minho¹; Jun, Chang-Duk¹; Lee, Sangho^{2^†}; Park, Jihwan^1,†

¹School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.

²Division of Nephrology, Department of Internal Medicine, Kyung Hee University, Seoul, Republic of Korea

^*These authors contributed equally to this work as the first authors.

^†Correspondence: Dr. Jihwan Park, Sangho Lee

Abstract

Background: Accumulated evidence demonstrates that long non-coding RNAs (lncRNAs) regulate cell differentiation and homeostasis, influencing kidney aging and disease. Despite their versatility, the function of lncRNA remains poorly understood due to the lack of a reference map of lncRNA transcriptome in various cell types.

Methods: In this study, we employed a targeted single-cell RNA sequencing (scRNA-seq) method to enrich and characterize lncRNAs in individual cells. We applied this method to various mouse tissues, including normal and aged kidneys.

Results: Through tissue-specific clustering analysis, we identified cell type-specific lncRNAs that showed a high correlation with known cell-type marker genes. Furthermore, we constructed gene regulatory networks (GRNs) to explore the functional roles of differentially expressed lncRNAs in each cell type. In the kidney, we observed dynamic expression changes of lncRNAs during aging, with specific changes in glomerular cells. These cell type- and age-specific expression patterns of lncRNAs provide insights into their potential roles in regulating cellular processes, such as immune response and energy metabolism, during kidney aging.

Conclusions: Our study sheds light on the comprehensive landscape of lncRNA expression and function and provides a valuable resource for future analysis of lncRNAs (https://gist-fgl.github.io/sc-lncrna-atlas/).

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-16)
연구진국내 연구진
분야 생명과학 > 분자생물학

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