한빛사논문
- 조회344
Quang Luu Quoc, MD, PhD,a,b* YeJi Kim, BS,b,c* Gunwoo Park, BS,a,b* Thi Bich Tra Cao, MD, PhD,a,b, Youngwoo Choi, PhD,d Yong Hwan Park, PhD,b,c‡ Hae-Sim Park, MD, PhDa,b‡
From aDepartment of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Korea;
bDepartment of Biomedical Sciences, Ajou University School of Medicine, Suwon, Korea;
cDepartment of Microbiology, Ajou University School of Medicine, Suwon, Korea;
dDepartment of Biomaterials Science (BK21 FOUR Program), College of Natural Resources and Life Science, Pusan National University, Miryang, Korea
*These authors contributed equally to this work as first authors.
‡These authors contributed equally to this work as corresponding authors
Abstract
Background: Neutrophilic asthma (NA) is a severe asthma phenotype associated with steroid resistance and IL-1β overproduction; however, the exact mechanism remains unclear. Moreover, the dysfunction of tumor necrosis factor-alpha signaling pathway, a regulator of IL-1β production, was associated with the deficiency of OTU deubiquitinase with linear linkage specificity (otulin) in autoimmune patients.
Objective: We hypothesized that otulin downregulation in macrophages (Mφ) could trigger Mφ activation via the nucleotide-binding domain, leucine-rich repeat, and pyrin domain-containing protein 3 (NLRP3) inflammasome signaling pathway.
Methods: We assessed the expressions of otulin in blood monocyte subsets from NA patients and in alveolar Mφ from NA mice. Additionally, we evaluated the functional consequences of otulin deficiency in bone marrow-derived Mφ (BMDMs). The effects of inhibiting receptor-interacting protein kinase (RIPK)-1 and RIPK-3 on neutrophils and group 3 innate lymphoid cells (ILC3s) were assessed in vitro and in vivo.
Results: When comparing non-classical monocytes, a significant downregulation of otulin in the intracellular components was observed in NA patients when compared to healthy controls (P = 0.005). Additionally, isolated alveolar Mφ from the NA mice exhibited lower otulin expression compared to those from control mice. Following otulin knockdown in BMDMs, we observed spontaneous IL-1β production depending on NLRP3 inflammasome. Moreover, the infiltrated neutrophils and ILC3s were significantly decreased by combined treatment of RIPK-1 and RIPK-3 inhibitors through blocking IL-1β release in NA.
Conclusions: Our findings suggest that IL-1β overproduction caused by a deficiency of otulin, an upstream triggering factor, could be a promising diagnostic and therapeutic target for NA.
논문정보
- Research article
- 2024년 04월 (BRIC 등록일 2024-04-11)
- 국내 연구진
- 의약학 > 면역의학
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