이혜진 (University of Wisconsin-Madison, 현 University of Michigan) | 한빛사논문 > 한빛사

한빛사논문

조회194

이혜진Hye Jin Lee

University of Wisconsin-Madison, 현 University of Michigan

Sci. Adv., Apr 12 2024, 10 (15) | https://doi.org/10.1126/sciadv.adj1444 [64Cu]Cu-PEG-FUD peptide for noninvasive and sensitive detection of murine pulmonary fibrosis

Hye Jin Lee^1†‡, Ksenija Bernau^2†*, Thomas J. Harr², Zachary T. Rosenkrans³, Grace A. Kessler², Kristen Stott², Angie Tebon Oler², Babita Rahar², Terry Zhu², Yadira Medina-Guevara³, Nikesh Gupta¹, Inyoung Cho¹, Metti K. Gari⁴, Brian M. Burkel⁴, Justin J. Jeffery⁵, Ashley M. Weichmann⁵, Bianca R. Tomasini-Johansson^2,6, Suzanne M. Ponik^4,5, Jonathan W. Engle³, Reinier Hernandez^3,5, Glen S. Kwon^1,5§, Nathan Sandbo^2§

¹Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA.

²Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, 600 Highland Avenue, Madison, WI 53792, USA.

³Department of Medical Physics, School of Medicine and Public Health, University of Wisconsin-Madison, 1111 Highland Avenue, Madison, WI 53705, USA.

⁴Department of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, 1111 Highland Avenue, Madison, WI 53705, USA.

⁵University of Wisconsin Carbone Cancer Center, University of Wisconsin-Madison, 1111 Highland Avenue, Madison, WI, USA.

⁶Arrowhead Pharmaceuticals, 502 S. Rosa Rd., Madison, WI 53719, USA.

^*corresponding author : Ksenija Bernau

^†these authors contributed equally to this work.

^‡Present address: department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, Mi 48109, USA.

^§these authors contributed equally to this work.

Abstract

Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease resulting in irreversible scarring within the lungs. However, the lack of biomarkers that enable real-time assessment of disease activity remains a challenge in providing efficient clinical decision-making and optimal patient care in IPF. Fibronectin (FN) is highly expressed in fibroblastic foci of the IPF lung where active extracellular matrix (ECM) deposition occurs. Functional upstream domain (FUD) tightly binds the N-terminal 70-kilodalton domain of FN that is crucial for FN assembly. In this study, we first demonstrate the capacity of PEGylated FUD (PEG-FUD) to target FN deposition in human IPF tissue ex vivo. We subsequently radiolabeled PEG-FUD with ⁶⁴Cu and monitored its spatiotemporal biodistribution via μPET/CT imaging in mice using the bleomycin-induced model of pulmonary injury and fibrosis. We demonstrated [ ⁶⁴Cu]Cu-PEG-FUD uptake 3 and 11 days following bleomycin treatment, suggesting that radiolabeled PEG-FUD holds promise as an imaging probe in aiding the assessment of fibrotic lung disease activity.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-18)
연구진국외 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기