조채환 (중앙대학교) | 한빛사논문 > 한빛사

한빛사논문

조회561

조채환Chae Hwan Cho

중앙대학교

CV File 177 kb

Biosens. Bioelectron., Apr 02 2024, 255 S0956-5663(24)00274-4 | https://doi.org/10.1016/j.bios.2024.116269 Nanozyme-assisted molecularly imprinted polymer-based indirect competitive ELISA for the detection of marine biotoxin

Chae Hwan Cho^a, Ji Hong Kim ^a, Navnath S. Padalkar ^a, Y. Veera Manohara Reddy ^b, Tae Jung Park ^c, Jinyoung Park ^d, Jong Pil Park ^a

^aDepartment of Food Science and Technology, GreenTech-Based Food Safety Research Group (BK21 Four), Chung-Ang University, Anseong, 17546, Republic of Korea

^bDepartment of Chemistry, Sri Venkateswara College, University of Delhi, New Delhi, India

^cDepartment of Chemistry, Institute of Interdisciplinary Convergence Research, Research Institute of Chem-Bio Diagnostic Technology, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06974, Republic of Korea

^dDepartment of Polymer Science & Engineering, Kyungpook National University, 80 Daehak-ro, Daegu, 41566, Republic of Korea

Corresponding author : Jong Pil Park

Abstract

Saxitoxin (STX), which is produced by certain dinoflagellate species, is a type of paralytic shellfish poisoning toxin that poses a serious threat to human health and the environment. Therefore, developing a technology for the convenient and cost-effective detection of STX is imperative. In this study, we developed an affinity peptide-imprinted polymer-based indirect competitive ELISA (ic-ELISA) without using enzyme-toxin conjugates. AuNP/Co ₃O ₄@Mg/Al cLDH was synthesized by calcining AuNP/ZIF-67@Mg/Al LDH, which was obtained by combining AuNPs, ZIF-67, and flower-like Mg/Al LDH. This synthesized nanozyme exhibited high catalytic activity (K _m = 0.24 mM for TMB and 132.5 mM for H ₂O ₂). The affinity peptide-imprinted polymer (MIP) was imprinted with an STX-specific template peptide (STX MIP) on a multi-well microplate and then reacted with an STX-specific signal peptide (STX SP). The interaction between the STX SP and MIP was detected using a streptavidin-coated nanozyme (SA-AuNP/Co ₃O ₄@Mg/Al cLDH). The developed MIP-based ic-ELISA exhibited excellent selectivity and sensitivity, with a limit of detection of 3.17 ng/mL (equivalent: 0.317 μg/g). Furthermore, the system was validated using a commercial ELISA kit and mussel tissue samples, and it demonstrated a high STX recovery with a low coefficient of variation. These results imply that the developed ic-ELISA can be used to detect STX in real samples.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-08)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

댓글 작성 로그인

CV 열람하기

연락처 보기