이정환 (단국대학교 조직재생공학연구원/치과대학/나노바이오의과학과)

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단국대학교 조직재생공학연구원/치과대학/나노바이오의과학과

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Adv. Healthc. Mater., Apr 03 2024, | https://doi.org/10.1002/adhm.202400043 Development of an Injectable Biphasic Hyaluronic Acid-based Hydrogel with Stress Relaxation Properties for Cartilage Regeneration

Han-Sem Kim ^1,2, Cheng Ji Li ^1,2, Sung-Min Park ^1,2, Kyung Wook Kim ³, Ji-Hun Mo ⁴, Guang-Zhen Jin ^1,2,5, Hae-Hyoung Lee^1,2,5,6,7, Hae-Won Kim ^1,2,5,6,7, Ueon Sang Shin^1,2, Jung-Hwan Lee ^1,2,5,6,7

¹Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, 31116, Republic of Korea.

²Department of Nano-biomedical Science & BK21 FOUR NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 31116, Republic of Korea.

³Department of Orthopaedic Surgery, Dankook University Hospital, Cheonan, 31116, Republic of Korea.

⁴Department of Otorhinolaryngology, 464237, Dankook University College of Medicine, Cheonan, 31116, Republic of Korea.

⁵Cell & Matter Institute, Dankook University, Cheonan, 31116, Republic of Korea.

⁶Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, 31116, South Korea.

⁷UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, 31116, South Korea.

H-. S. Kim and C. J. Li equally contributed as co-first authors.

CORRESPONDING AUTHORS : Hae-Won Kim, Ueon Sang Shin, Jung-Hwan Lee

Abstract

Biomimetic stress-relaxing hydrogels with reversible crosslinks have attracted significant attention for stem cell tissue regeneration compared with elastic hydrogels. However, stress-relaxing hyaluronic acid (HA)-based hydrogels fabricated using conventional technologies lack stability, biocompatibility, and mechanical tunability. Here, we aim to address these challenges by incorporating calcium or phosphate components into the HA backbone, which allows reversible crosslinking of HA with alginate to form interpenetrating networks, offering stability and mechanical tunability for mimicking cartilage. Diverse stress-relaxing hydrogels (τ1/2; SR50, 60-2000 s) were successfully prepared at ∼3 kPa stiffness with self-healing and shear-thinning abilities, favoring hydrogel injection. In vitro cell experiments with RNA sequencing analysis demonstrate that hydrogels tune chondrogenesis in a biphasic manner (hyaline or calcified) depending on the stress-relaxation properties and phosphate components. In vivo studies confirm the potential for biphasic chondrogenesis. These results indicate that the proposed stress-relaxing HA-based hydrogel with biphasic chondrogenesis (hyaline or calcified) is a promising material for cartilage regeneration. This article is protected by copyright. All rights reserved.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-09)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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