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Stand Up Therapeutics

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Nano Lett., Apr 01 2024, | https://doi.org/10.1021/acs.nanolett.4c00451 Plasmonic Annular Nanotrenches with 1 nm Nanogaps for Detection of SARS-CoV-2 Using SERS-Based Immunoassay

Sungwoo Lee ^1,2, Seonghyeon Lee ³, Woongkyu Park ⁴, Soohyun Lee ¹, Sunwoo Kwon ¹, Myeong Jin Oh ¹, MohammadNavid Haddadnezhad ¹, Insub Jung^1,2, Bohyeon Kim ³, Jieun Park ³, Kyu Sung Shin⁵, Hyungdon Lee ⁶, Junsang Yoo ^7,8, Won-Keun Kim ^3,9, Sungho Park ¹⁰

¹Department of Chemistry, Sungkyunkwan University, Suwon 16419, Republic of Korea.

²Institute of Basic Science, Sungkyunkwan University, Suwon 16419, Republic of Korea.

³Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, Republic of Korea.

⁴Photonics Energy Components Research Center, Korea Photonics Technology Institute (KOPTI), Gwangju 61007, Republic of Korea.

⁵Laboratory Medicine, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon 24253, Republic of Korea.

⁶Department of Internal Medicine, College of Medicine, Hallym University, Chuncheon Sacred Heart Hospital, Chuncheon 24253, Republic of Korea.

⁷Department of Molecular Biology, Nuturn Science, Seoul 04418, Republic of Korea.

⁸Laboratory of Regenerative Medicine for Neurodegenerative Disease, Stand Up Therapeutics, Seoul 04418, Republic of Korea.

⁹Institute of Medical Research, College of Medicine, Hallym University, Chuncheon 24252, Republic of Korea.

¹⁰Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.

Corresponding Authors : Junsang Yoo, Won-Keun Kim, Sungho Park

Abstract

This study represents the synthesis of a novel class of nanoparticles denoted as annular Au nanotrenches (AANTs). AANTs are engineered to possess embedded, narrow circular nanogaps with dimensions of approximately 1 nm, facilitating near-field focusing for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via a surface-enhanced Raman scattering (SERS)-based immunoassay. Notably, AANTs exhibited an exceedingly low limit of detection (LOD) of 1 fg/mL for SARS-CoV-2 spike glycoproteins, surpassing the commercially available enzyme-linked immunosorbent assay (ELISA) by 6 orders of magnitude (1 ng/mL from ELISA). To assess the real-world applicability, a study was conducted on 50 clinical samples using an SERS-based immunoassay with AANTs. The results revealed a sensitivity of 96% and a selectivity of 100%, demonstrating the significantly enhanced sensing capabilities of the proposed approach in comparison to ELISA and commercial lateral flow assay kits.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-04-02)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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