진용수 (University of Illinois, Urbana-Champaign) | 한빛사논문 > 한빛사

한빛사논문

조회315

University of Illinois, Urbana-Champaign

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Bioresour. Technol., Mar 27 2024, S0960-8524(24)00344-4 | https://doi.org/10.1016/j.biortech.2024.130641 Self-Buffering system for Cost-Effective production of lactic acid from glucose and xylose using Acid-Tolerant Issatchenkia orientalis

Ye-Gi Lee ^a,b,1, Nam Kyu Kang ^c,1, Chanwoo Kim ^b,d, Vinh G. Tran ^b,e, Mingfeng Cao ^b,e, Yasuo Yoshikuni ^f, Huimin Zhao ^b,e, Yong-Su Jin^b,d

^aDepartment of Bio and Fermentation Convergence Technology and Center for Bioconvergence, Kookmin University, Seoul 02707, Korea

^bCarl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

^cDepartment of Chemical Engineering, College of Engineering, Kyung Hee University, Yongin, 17104, Republic of Korea

^dDepartment of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

^eDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

^fUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA

¹These authors contributed equally.

Corresponding author : Yong-Su Jin

Abstract

This study presents a cost-effective strategy for producing organic acids from glucose and xylose using the acid-tolerant yeast, Issatchenkia orientalis. I. orientalis was engineered to produce lactic acid from xylose, and the resulting strain, SD108XL, successfully converted sorghum hydrolysates into lactic acid. In order to enable low-pH fermentation, a self-buffering strategy, where the lactic acid generated by the SD108XL strain during fermentation served as a buffer, was developed. As a result, the SD108 strain produced 67 g/L of lactic acid from 73 g/L of glucose and 40 g/L of xylose, simulating a sugar composition of sorghum biomass hydrolysates. Moreover, techno-economic analysis (TEA) underscored the efficiency of the self-buffering strategy in streamlining the downstream process, thereby reducing production costs. These results demonstrate the potential of I. orientalis as a platform strain for the cost-effective production of organic acids from cellulosic hydrolysates.

논문정보

형식Research article
게재일2024년 03월 (BRIC 등록일 2024-04-02)
연구진국내+국외 연구진
분야 생명과학 > 생물공학

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