정수웅 (경희대학교)

한빛사논문

조회207

정수웅Su Woong Jung

경희대학교

Kidney Int., Mar 28 2024, S0085-2538(24)00244-8 | https://doi.org/10.1016/j.kint.2024.01.045 Single-cell lineage tracing approaches to track kidney cell development and maintenance

Baul Yoon¹, Hayoung Kim ¹, Su Woong Jung ^2,3, Jihwan Park ¹

¹School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea

²Division of Nephrology, Department of Internal Medicine, College of Medicine, Kyung Hee University, Seoul, Republic of Korea

³Division of Nephrology, Department of Internal Medicine, Kyung Hee University Hospital at Gangdong, Seoul, Republic of Korea

Correspondence: Su Woong Jung, Jihwan Park

Abstract

The kidney is a complex organ consisting of various cell types. Previous studies have aimed to elucidate the cellular relationships among these cell types in developing and mature kidneys using Cre-loxP-based lineage tracing. However, this methodology falls short of fully capturing the heterogeneous nature of the kidney, making it less than ideal for comprehensively tracing cellular progression during kidney development and maintenance. Recent technological advancements in single-cell genomics have revolutionized lineage tracing methodologies. Single-cell lineage tracing enables the simultaneous tracing of multiple cell types within complex tissues and their transcriptomic profiles, thereby allowing for the reconstruction of their lineage tree with cell state information. While single-cell lineage tracing has been successfully applied to investigate cellular hierarchies in various organs and tissues, its application in kidney research is currently lacking. This review comprehensively consolidates the single-cell lineage tracing methodologies, divided into four categories (CRISPR/Cas9-based, transposon-based, Polylox-based, and native barcoding methods), and outlines their technical advantages and disadvantages. Furthermore, we propose potential future research topics in kidney research that could benefit from single-cell lineage tracing and suggest suitable technical strategies to apply to these topics.

논문정보

형식Review Paper
게재일2024년 03월 (BRIC 등록일 2024-04-01)
연구진국내 연구진
분야 생명과학 > 분자생물학

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