민성진 (연세대학교)

한빛사논문

조회403

연세대학교

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Nat. Commun., Mar 22 2024, 15 (1) 2564 | https://doi.org/10.1038/s41467-024-46928-y Versatile human cardiac tissues engineered with perfusable heart extracellular microenvironment for biomedical applications

Sungjin Min ^1,15, Suran Kim ^1,2,15, Woo-Sup Sim ^3,4,15, Yi Sun Choi ¹, Hyebin Joo ¹, Jae-Hyun Park^3,4, Su-Jin Lee ⁵, Hyeok Kim ^3,4, Mi Jeong Lee ¹, Inhea Jeong ⁶, Baofang Cui ¹, Sung-Hyun Jo ⁷, Jin-Ju Kim ^3,4, Seok Beom Hong ⁸, Yeon-Jik Choi ⁹, Kiwon Ban ¹⁰, Yun-Gon Kim ⁷, Jang-Ung Park ^6,11,12,13, Hyang-Ae Lee ⁵, Hun-Jun Park ^3,4,14,* & Seung-Woo Cho ^1,2,12,13,*

¹Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
²Cellartgen, Seoul 03722, Republic of Korea.
³Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
⁴Division of Cardiology, Department of Internal Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul 06591, Republic of Korea.
⁵Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea.
⁶Department of Materials Science and Engineering, Yonsei University, Seoul 03722, Republic of Korea.
⁷Department of Chemical Engineering, Soongsil University, Seoul 06978, Republic of Korea.
⁸Department of Thoracic and Cardiovascular Surgery, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
⁹Division of Cardiology, Department of Internal Medicine, Eunpyeong St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea.
¹⁰Department of Biomedical Sciences, City University of Hong Kong, Kowloon 999077, Hong Kong.
¹¹Department of Neurosurgery, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
¹²Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea.
¹³Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul 03722, Republic of Korea.
¹⁴Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
¹⁵These authors contributed equally: Sungjin Min, Suran Kim, Woo-Sup Sim.

^*Corresponding authors: correspondence to Hun-Jun Park or Seung-Woo Cho

Abstract

Engineered human cardiac tissues have been utilized for various biomedical applications, including drug testing, disease modeling, and regenerative medicine. However, the applications of cardiac tissues derived from human pluripotent stem cells are often limited due to their immaturity and lack of functionality. Therefore, in this study, we establish a perfusable culture system based on in vivo-like heart microenvironments to improve human cardiac tissue fabrication. The integrated culture platform of a microfluidic chip and a three-dimensional heart extracellular matrix enhances human cardiac tissue development and their structural and functional maturation. These tissues are comprised of cardiovascular lineage cells, including cardiomyocytes and cardiac fibroblasts derived from human induced pluripotent stem cells, as well as vascular endothelial cells. The resultant macroscale human cardiac tissues exhibit improved efficacy in drug testing (small molecules with various levels of arrhythmia risk), disease modeling (Long QT Syndrome and cardiac fibrosis), and regenerative therapy (myocardial infarction treatment). Therefore, our culture system can serve as a highly effective tissue-engineering platform to provide human cardiac tissues for versatile biomedical applications.

논문정보

형식Research article
게재일2024년 03월 (BRIC 등록일 2024-03-26)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오소재

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