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Phytomedicine, Feb 13 2024, 128 S0944-7113(24)00114-4 | https://doi.org/10.1016/j.phymed.2024.155449
Identification of Peucedanum japonicum Thunb. extract components and their protective effects against dexamethasone-induced muscle atrophy
Young In Kim a,b, Hyunjung Lee a, Min Jung Kim a, Chang Hwa Jung a,c, Young-Soo Kim b, Jiyun Ahn a,c
aAging and Metabolism Research Group, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, South Korea
bDepartment of Food Science and Technology, Jeonbuk National University, Jeonju-si, South Korea
cDepartment of Food Biotechnology, Korea National University of Science and Technology, Daejeon, South Korea
Corresponding author : Jiyun Ahn
Abstract
Peucedanum japonicum Thunb. (PJ) is a vegetable widely consumed in East Asia and is known to have anticancer and anti-inflammatory effects. However, the effect of PJ on muscle atrophy remains elusive.
This study aimed to investigate the effect of PJ and its active compound on dexamethasone (DEX)-induced muscle atrophy.
We performed qualitative and quantitative analysis of PJ using ultra-performance liquid chromatography-mass spectrometry tandem mass spectrometry (UPLC-MS/MS) and high-performance liquid chromatography (HPLC), respectively. The efficacy of PJ and its main compound 4-caffeoylquinic acid (CQA) on muscle atrophy was evaluated in DEX-induced myotube atrophy and DEX-induced muscle atrophy in mouse myoblasts (C2C12) and C57BL/6 mice, in vitro and in vivo, respectively.
The UPLC-MS/MS and HPLC data showed that the concentration of 4-CQA in PJ was 18.845 mg/g. PJ and 4-CQA treatments significantly inhibited DEX-induced myotube atrophy by decreasing protein synthesis and glucocorticoid translocation to the nucleus in C2C12 myotubes. In addition, PJ enhanced myogenesis by upregulating myogenin and myogenic differentiation 1 in C2C12 cells. PJ supplementation effectively increased muscle function and mass, downregulated atrogenes, and decreased proteasome activity in C57BL/6 mice. Additionally, PJ effectively decreased the nuclear translocation of forkhead transcription factor 3 alpha by inhibiting glucocorticoid receptor.
Overall, PJ and its active compound 4-CQA alleviated skeletal muscle atrophy by inhibiting protein degradation. Hence, our findings present PJ as a potential novel pharmaceutical candidate for the treatment of muscle atrophy.
논문정보
- Research article
- 2024년 02월 (BRIC 등록일 2024-03-29)
- 국내 연구진
- 생명과학 > 세포생물학
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