장성호 (인천대학교)

한빛사논문

조회274

인천대학교

CV File 218 kb

Biotechnol. Adv., Mar 21 2024, S0734-9750(24)00037-5 | https://doi.org/10.1016/j.biotechadv.2024.108343 Mobile genetic element-based gene editing and genome engineering: Recent advances and applications

Jaeseong Hwang ^a,1, Dae-Yeol Ye ^b,1, Gyoo Yeol Jung^a,b, Sungho Jang ^c,d,e

^aSchool of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea

^bDepartment of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea

^cDepartment of Bioengineering and Nano-Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea

^dDivision of Bioengineering, College of Life Sciences and Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea

^eResearch Center for Bio Materials & Process Development, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors: Gyoo Yeol Jung, Sungho Jang

Abstract

Genome engineering has revolutionized several scientific fields, ranging from biochemistry and fundamental research to therapeutic uses and crop development. Diverse engineering toolkits have been developed and used to effectively modify the genome sequences of organisms. However, there is a lack of extensive reviews on genome engineering technologies based on mobile genetic elements (MGEs), which induce genetic diversity within host cells by changing their locations in the genome. This review provides a comprehensive update on the versatility of MGEs as powerful genome engineering tools that offers efficient solutions to challenges associated with genome engineering. MGEs, including DNA transposons, retrotransposons, retrons, and CRISPR-associated transposons, offer various advantages, such as a broad host range, genome-wide mutagenesis, efficient large-size DNA integration, multiplexing capabilities, and in situ single-stranded DNA generation. We focused on the components, mechanisms, and features of each MGE-based tool to highlight their cellular applications. Finally, we discussed the current challenges of MGE-based genome engineering and provided insights into the evolving landscape of this transformative technology. In conclusion, the combination of genome engineering with MGE demonstrates remarkable potential for addressing various challenges and advancing the field of genetic manipulation, and promises to revolutionize our ability to engineer and understand the genomes of diverse organisms.

논문정보

형식Review Paper
게재일2024년 03월 (BRIC 등록일 2024-03-25)
연구진국내 연구진
분야 생명과학 > 생물공학

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