박창진 (세종대학교)

한빛사논문

조회721

세종대학교

Plant J., Mar 20 2024, | https://doi.org/10.1111/tpj.16694 Rice small secreted peptide, OsRALF26, recognized by FERONIA-like receptor 1 induces immunity in rice and Arabidopsis

Oh-Kyu Kwon¹, Hyeran Moon^1,†, A-Ram Jeong¹, Gunn Yeom² and Chang-Jin Park^1,2,*

¹Department of Molecular Biology, Sejong University, Seoul 05006, South Korea,

²Department of Bioresources Engineering, Sejong University, Seoul 05006, South Korea

^*For correspondence : Chang-Jin Park

^†Present address: Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 55108, USA

Abstract

Rapid alkalinization factors (RALFs), belonging to a family of small secreted peptides, have been considered as important signaling molecules in diverse biological processes, including immunity. Current studies on RALF-modulated immunity mainly focus on Arabidopsis, but little is reported in crop plants. The rice immune receptor XA21 confers immunity to the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo). Here, we pursued functional characterization of rice RALF26 (OsRALF26) up-regulated by Xoo during XA21-mediated immune response. When applied exogenously as a recombinant peptide, OsRALF26 induced a series of immune responses, including pathogenesis-related genes (PRs) induction, reactive oxygen species (ROS) production, and callose deposition in rice and/or Arabidopsis. Transgenic rice and Arabidopsis overexpressing OsRALF26 exhibited significantly enhanced resistance to Xoo and Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), respectively. In yeast two-hybrid, pull-down assays, and co-immunoprecipitation analyses, rice FER-like receptor 1 (OsFLR1) was identified as a receptor of OsRALF26. Transient expression of OsFLR1 in Nicotiana benthamiana leaves displayed significantly increased ROS production and callose deposition after OsRALF26 treatment. Together, we propose that OsRALF26 induced by Xoo in an XA21-dependent manner is perceived by OsFLR1 and may play a novel role in the enforcement of XA21-mediated immunity.

논문정보

형식Research article
게재일2024년 03월 (BRIC 등록일 2024-03-28)
연구진국내 연구진
분야 생명과학 > 식량작물 및 원예작물

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