이승구 (한국생명공학연구원, UST) | 한빛사논문 > 한빛사

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한국생명공학연구원, UST

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Bioresour. Technol., Mar 16 2024, S0960-8524(24)00310-9 | https://doi.org/10.1016/j.biortech.2024.130607 Unlocking synergies: Harnessing the potential of biological methane sequestration through metabolic coupling between Methylomicrobium alcaliphilum 20Z and Chlorella sp. HS2

Jin-Ho Yun ^a,b,c,1, Hyewon Lee ^d,e,1, Jang-Won Nam ^a,c, Minji Ko ^d,e, Jaehyun Park ^a, Dae-Hee Lee ^b,d,e, Seung-Goo Lee^d,e, Hee-Sik Kim ^a,c

^aCell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea

^bDepartment of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, Republic of Korea

^cDepartment of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science & Technology (UST), Daejeon 34113, Republic of Korea

^dSynthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea

^eDepartment of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science & Technology (UST), Daejeon 34113, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors : Seung-Goo Lee, Hee-Sik Kim

Abstract

A halotolerant consortium between microalgae and methanotrophic bacteria could effectively remediate in situ CH ₄ and CO ₂, particularly using saline wastewater sources. Herein, Methylomicrobium alcaliphilum 20Z was demonstrated to form a mutualistic association with Chlorella sp. HS2 at a salinity level above 3.0%. Co-culture significantly enhanced the growth of both microbes, independent of initial inoculum ratios. Additionally, increased methane provision in enclosed serum bottles led to saturated methane removal. Subsequent analyses suggested nearly an order of magnitude increase in the amount of carbon sequestered in biomass in methane-fed co-cultures, conditions that also maintained a suitable cultural pH suitable for methanotrophic growth. Collectively, these results suggest a robust metabolic coupling between the two microbes and the influence of the factors other than gaseous exchange on the assembled consortium. Therefore, multi-faceted investigations are needed to harness the significant methane removal potential of the identified halotolerant consortium under conditions relevant to real-world operation scenarios.

논문정보

형식Research article
게재일2024년 03월 (BRIC 등록일 2024-04-02)
연구진국내 연구진
분야 생명과학 > 생물공학

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