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J. Nanobiotechnol., Mar 17 2024, 22 (1) | https://doi.org/10.1186/s12951-024-02364-0
scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticles
Haribalan Perumalsamy1,2,3,6, Xiao Xiao3, Hyun-Yi Kim4 and Tae-Hyun Yoon2,3,5,6*
1Center for Creative Convergence Education, Hanyang University, Seoul 04763, Republic of Korea
2Institute of Next Generation Material Design, Hanyang University, Seoul 04763, Republic of Korea
3Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea
4NGeneS Inc, 362, Gwangdeok 1-ro, Sangnok-gu, Ansan-si, Gyeonggi-do 15495, Republic of Korea
5Department of Medical and Digital Engineering, Hanyang University, Seoul 04763, Republic of Korea
6Research Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Republic of Korea
*Correspondence: Tae-Hyun Yoon
Abstract
The assessment of AgNPs toxicity in vitro and in vivo models are frequently conflicting and inaccurate. Nevertheless, single cell immunological responses in a heterogenous environment have received little attention. Therefore, in this study, we have performed in-depth analysis which clearly revealed cellular-metal ion association as well as specific immunological response. Our study didn't show significant population differences in PMBC between control and AgNPs group implying no toxicological response. To confirm it further, deep profiling identified differences in subsets and differentially expressed genes (DEGs) of monocytes, B cells and T cells. Notably, monocyte subsets showed significant upregulation of metallothionein (MT) gene expression such as MT1G, MT1X, MT1E, MT1A, and MT1F. On the other hand, downregulation of pro-inflammatory genes such as IL1β and CCL3 in both CD16 + and CD16- monocyte subsets were observed. This result indicated that AgNPs association with monocyte subsets de-promoted inflammatory responsive genes suggesting no significant toxicity observed in AgNPs treated group. Other cell types such as B cells and T cells also showed negligible differences in their subsets suggesting no toxicity response. Further, AgNPs treated group showed upregulation of cell proliferation, ribosomal synthesis, downregulation of cytokine release, and T cell differentiation inhibition. Overall, our results conclude that treatment of AgNPs to PMBC cells didn't display immunological related cytotoxicity response and thus motivate researchers to use them actively for biomedical applications.
논문정보
- Research article
- 2024년 03월 (BRIC 등록일 2024-03-20)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
관련 웨비나
![scRNA-seq 분석 결과 은 나노입자에 노출된 PMBC에서 면역조절 의존성 염증반응의 억제가 발견 [J. Nanobiotechnol.]](/upload/board/files/onseminar/thumb/thumb_0000712.png)
학술웨비나
scRNA-seq 분석 결과 은 나노입자에 노출된 PMBC에서 면역조절 의존성 염증반응의 억제가 발견 [J. Nanobiotechnol.] scRNA-seq analysis discovered suppression of immunomodulatory dependent inflammatory response in PMBCs exposed to silver nanoparticles The assessment of AgNPs toxicity in vitro and in vivo models are frequently conflicting and inaccurate. Nevertheless, single cell immunological responses in a heterogenous environment have received little attention. Therefore, in this study, we have performed in-depth analysis which clearly revealed cellular-metal ion association as well as specific immunological response. Our study didn’t show significant population differences in PMBC between control and AgNPs group implying no toxicological response. To confirm it further, deep profiling identified differences in subsets and differentially expressed genes (DEGs) of monocytes, B cells and T cells. Notably, monocyte subsets showed significant upregulation of metallothionein (MT) gene expression such as MT1G, MT1X, MT1E, MT1A, and MT1F. On the other hand, downregulation of pro-inflammatory genes such as IL1β and CCL3 in both CD16 + and CD16- monocyte subsets were observed. This result indicated that AgNPs association with monocyte subsets de-promoted inflammatory responsive genes suggesting no significant toxicity observed in AgNPs treated group. Other cell types such as B cells and T cells also showed negligible differences in their subsets suggesting no toxicity response. Further, AgNPs treated group showed upregulation of cell proliferation, ribosomal synthesis, downregulation of cytokine release, and T cell differentiation inhibition. Overall, our results conclude that treatment of AgNPs to PMBC cells didn’t display immunological related cytotoxicity response and thus motivate researchers to use them actively for biomedical applications.- Haribalan Perumalsamy(한양대학교)
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