한빛사논문
- 조회334
Donghui Choe 1, Connor A. Olson 1, Richard Szubin 1, Hannah Yang 1, Jaemin Sung 1, Adam M. Feist 1,2 & Bernhard O. Palsson 1,2,*
1Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA.
2Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Copenhagen, Denmark.
*Corresponding author: correspondence to Bernhard O. Palsson
Abstract
Machine learning applied to large compendia of transcriptomic data has enabled the decomposition of bacterial transcriptomes to identify independently modulated sets of genes, such iModulons represent specific cellular functions. The identification of iModulons enables accurate identification of genes necessary and sufficient for cross-species transfer of cellular functions. We demonstrate cross-species transfer of: 1) the biotransformation of vanillate to protocatechuate, 2) a malonate catabolic pathway, 3) a catabolic pathway for 2,3-butanediol, and 4) an antimicrobial resistance to ampicillin found in multiple Pseudomonas species to Escherichia coli. iModulon-based engineering is a transformative strategy as it includes all genes comprising the transferred cellular function, including genes without functional annotation. Adaptive laboratory evolution was deployed to optimize the cellular function transferred, revealing mutations in the host. Combining big data analytics and laboratory evolution thus enhances the level of understanding of systems biology, and synthetic biology for strain design and development.
논문정보
- Research article
- 2024년 03월 (BRIC 등록일 2024-03-18)
- 국외 연구진
- 생명과학 > 생물공학
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