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Adv. Sci., Mar 17 2024, | https://doi.org/10.1002/advs.202306630 AFM Imaging Reveals MicroRNA-132 to be a Positive Regulator of Synaptic Functions

Ikbum Park ^{1 *}, Hyun Jin Kim², Juyoung Shin², Yu Jin Jung ³, Donggyu Lee ⁴, Ji-Seon Lim ⁵, Jong Mok Park ¹, Joon Won Park ⁵, Joung-Hun Kim ^{2 6 *}

¹Technical Support Center for Chemical Industry, Korea Research Institute of Chemical Technology (KRICT), Ulsan, 44412, Republic of Korea.
²Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
³Center for Specialty Chemicals, Korea Research Institute of Chemical Technology (KRICT), Ulsan, 44412, Republic of Korea.
⁴Division of Electronics and Information System, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, 42988, Republic of Korea.
⁵Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.
⁶Institute of Convergence Science, Yonsei University, Seoul, 03722, Republic of Korea.

^†I.P. and H.J.K. contributed equally to this work.
^*Corresponding authors: correspondence to Ikbum Park or Joung-Hun Kim

Abstract

The modification of synaptic and neural connections in adults, including the formation and removal of synapses, depends on activity-dependent synaptic and structural plasticity. MicroRNAs (miRNAs) play crucial roles in regulating these changes by targeting specific genes and regulating their expression. The fact that somatic and dendritic activity in neurons often occurs asynchronously highlights the need for spatial and dynamic regulation of protein synthesis in specific milieu and cellular loci. MicroRNAs, which can show distinct patterns of enrichment, help to establish the localized distribution of plasticity-related proteins. The recent study using atomic force microscopy (AFM)-based nanoscale imaging reveals that the abundance of miRNA(miR)-134 is inversely correlated with the functional activity of dendritic spine structures. However, the miRNAs that are selectively upregulated in potentiated synapses, and which can thereby support prospective changes in synaptic efficacy, remain largely unknown. Using AFM force imaging, significant increases in miR-132 in the dendritic regions abutting functionally-active spines is discovered. This study provides evidence for miR-132 as a novel positive miRNA regulator residing in dendritic shafts, and also suggests that activity-dependent miRNAs localized in distinct sub-compartments of neurons play bi-directional roles in controlling synaptic transmission and synaptic plasticity.

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형식Research article
게재일2024년 03월 (BRIC 등록일 2024-03-18)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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