한빛사논문
- 조회164
전북대학교
Richa Jaswal a,b,c, Dinesh Kumar a,b,c, Vignesh Krishnamoorthi Kaliannagounder c,d, Abdelrahman I. Rezk b,e, Rupesh Kandel b,c, Chan Hee Park a,b, Kyung Hyun Min f
aDivision of Mechanical Design Engineering, Jeonbuk National University, Jeonju, 54896, South Korea
bDepartment of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, Jeonju, 54896, South Korea
cDepartment of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju, 54896, South Korea
dSchool of Engineering, Newcastle University, Newcastle Upon Tyne, NE1 7RU, United Kingdom
eDepartment of Physiology, Jeonbuk National University Medical School, Jeonju-si, 54907, South Korea
fSchool of Pharmacy, Jeonbuk National University, Jeonju, 54896, South Korea
Corresponding authors: Dinesh Kumar, Chan Hee Park, Kyung Hyun Min
Abstract
nanospheres (GNSs) were integrated into polycaprolactone (PCL) to fabricate bifunctional randomly oriented PCL-GNSs@PDA nanofibrous composite scaffolds and applied for photothermal bone cancer therapy and robust bone tissue regeneration. GNSs (30 nm sized) were coated with a uniform 10 nm layer of PDA, and the resulting GNSs@PDA core-shell nanoparticles were incorporated in three different concentrations (1.5, 3.0, and 5.0 mg) in PCL-based random nanofibers. The integration of GNSs@PDA nanoparticles enhanced electric conductivity, surface area, elasticity, compressive strength, photothermal properties as well as biocompatibility, photo-activity, and protein absorption of synthesized PCL-GNSs@PDA composite nanofibers which played a crucial role on its bifunctionality. Random-oriented PCL-GNSs@PDA (5.0 mg) nanofibrous scaffold showed superior photothermal activity which led to 94 % of bone cancer cell (MG-63 cells) ablation by maximum cell alteration and damaged cytoskeleton at low NIR light power (0.5 W/cm2) irradiation for 5 min. FACS analysis after 24 hours also displayed a higher percentage of apoptosis (60.2 %) in comparison to pure PCL (7.5 %) suggesting that PCL-GNSs@PDA (5.0 mg) induced highly efficient apoptosis in MG-63 cells. Also, PCL-GNSs@PDA (5.0 mg) showed higher cell proliferation, excellent cytocompatibility, and optimal cell adhesion for bone tissue (MC3T3-E1 cells) regeneration after 21-days as suggested by ALP, ARS, von Kossa staining, and qRT-PCR results. Bifunctional PCL-GNSs@PDA showed great potential as osteopromotive and bioinspired nanocomposite tissue engineering scaffolds for highly efficient bone cancer phototherapy and bone reconstruction.
논문정보
- Research article
- 2024년 03월 (BRIC 등록일 2024-03-13)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오소재
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