이평세 (Harvard Medical School / UC Berkeley)

한빛사논문

조회310

Harvard Medical School / UC Berkeley

CV File 956 kb

Sci. Adv., Mar 08 2024, 10 (10) eadm8597 | https://doi.org/10.1126/sciadv.adm8597 Acoustic separation and concentration of exosomes for nucleotide detection: ASCENDx

Ty D. Naquin^1†, Aidan J. Canning^2†, Yuyang Gu¹, Jianing Chen², Chloe M. Naquin¹, Jianping Xia¹, Brandon Lu¹, Shujie Yang¹, Aleksandra Koroza¹, Katherine Lin¹, Hsin-Neng Wang², William R. Jeck³, Luke P. Lee^4,5,6,7*, Tuan Vo-Dinh^2*, Tony Jun Huang^1*

¹department of Mechanical engineering and Materials Science, duke University, durham, Nc 27708, USA.

²department of Biomedical engineering, duke University, durham, Nc 27708, USA.

³department of Pathology, duke University Medical center, durham, Nc 27708, USA.

⁴harvard Medical School, harvard University; Renal division and division of engineering in Medicine, department of Medicine, Brigham and Women’s hospital, Boston, MA 02115, USA.

⁵department of Bioengi-neering and department of electrical engineering and computer Science, Uni-versity of california, Berkeley, Berkeley, cA 94720, USA.

⁶institute of Quantum Biophysics, department of Biophysics, Sungkyunkwan University, Suwon, Gyeonggi-do, Korea.

⁷department of chemistry and Nanoscience, ewha Womans University, Seoul, Korea.

^*corresponding authors: Luke P. Lee, Tuan Vo-Dinh, Tony Jun Huang

^†these authors contributed equally to this work.

Abstract

Efficient isolation and analysis of exosomal biomarkers hold transformative potential in biomedical applications. However, current methods are prone to contamination and require costly consumables, expensive equipment, and skilled personnel. Here, we introduce an innovative spaceship-like disc that allows Acoustic Separation and Concentration of Exosomes and Nucleotide Detection: ASCENDx. We created ASCENDx to use acoustically driven disc rotation on a spinning droplet to generate swift separation and concentration of exosomes from patient plasma samples. Integrated plasmonic nanostars on the ASCENDx disc enable label-free detection of enriched exosomes via surface-enhanced Raman scattering. Direct detection of circulating exosomal microRNA biomarkers from patient plasma samples by the ASCENDx platform facilitated a diagnostic assay for colorectal cancer with 95.8% sensitivity and 100% specificity. ASCENDx overcomes existing limitations in exosome-based molecular diagnostics and holds a powerful position for future biomedical research, precision medicine, and point-of-care medical diagnostics.

논문정보

형식Research article
게재일2024년 03월 (BRIC 등록일 2024-03-11)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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