강동민 (이화여자대학교)

한빛사논문

조회303

이화여자대학교

CV File 261 kb

Redox Biol., Feb 29 2024, 71 S2213-2317(24)00073-9 | https://doi.org/10.1016/j.redox.2024.103097 Control of the signaling role of PtdIns(4)P at the plasma membrane through H2O2-dependent inactivation of synaptojanin 2 during endocytosis

Su In Jo ^a,1, Suree Kim ^b,1, Jung Mi Lim ^c, Sue Goo Rhee^c, Bo-Gyeong Jeong ^d, Sun-Shin Cha ^d,e, Jae-Byum Chang ^f, Dongmin Kang^a,b

^aDepartment of Life Science, Ewha Womans University, Seoul, Republic of Korea

^bFluorescence Core Imaging Center and Bioimaging Data Curation Center, Ewha Womans University, Seoul, Republic of Korea

^cBiochemistry and Biophysics Center, NHLBI, National Institutes of Health, Bethesda, MD, 20892, USA

^dR&D Division, TODD PHARM CO. LTD., Seoul, Republic of Korea

^eDepartment of Chemistry & Nanoscience, Ewha Womans University, Seoul, Republic of Korea

^fDepartment of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea

¹These authors contributed equally to this work.

Corresponding author : Dongmin Kang

Abstract

Phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P ₂] is implicated in various processes, including hormone-induced signal transduction, endocytosis, and exocytosis in the plasma membrane. However, how H ₂O ₂ accumulation regulates the levels of PtdIns(4,5)P ₂ in the plasma membrane in cells stimulated with epidermal growth factors (EGFs) is not known. We show that a plasma membrane PtdIns(4,5)P ₂-degrading enzyme, synaptojanin (Synj) phosphatase, is inactivated through oxidation by H ₂O ₂. Intriguingly, H ₂O ₂ inhibits the 4-phosphatase activity of Synj but not the 5-phosphatase activity. In EGF-activated cells, the oxidation of Synj dual phosphatase is required for the transient increase in the plasma membrane levels of phosphatidylinositol 4-phosphate [PtdIns(4)P], which can control EGF receptor-mediated endocytosis. These results indicate that intracellular H ₂O ₂ molecules act as signaling mediators to fine-tune endocytosis by controlling the stability of plasma membrane PtdIns(4)P, an intermediate product of Synj phosphoinositide dual phosphatase.

논문정보

형식Research article
게재일2024년 02월 (BRIC 등록일 2024-03-07)
연구진국내(교신)+국외 연구진
분야 생명과학 > 세포생물학

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