유형원 (분당서울대학교병원) | 한빛사논문 > 한빛사

한빛사논문

조회394

분당서울대학교병원

CV File 308 kb

Int. J. Surg., Mar 04 2024, | https://doi.org/10.1097/JS9.0000000000001284 Analyzing circulating tumor cells and epithelial-mesenchymal transition status of papillary thyroid carcinoma patients following thyroidectomy: a prospective cohort study

Yu, Hyeong Won^a,b; Park, Eunju^c; Lee, Ja Kyung^a; Kim, Woochul^a; Kong, Ju Hyun^c; Sunoo, Joseph^c; Hong, Soon-Cheol^c; Choi, June Young^a,b

^aDepartment of Surgery, Seoul National University Bundang Hospital, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do, Korea

^bDepartment of Surgery, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul, Korea

^cCytoDx Inc., 331, Pangyo-ro, Bundan-gu, Seongnam, Gyeonggi-do, Korea

Hyeong Won Yu, Eunju Park These authors contributed equally to this work.

^*Corresponding author : Choi, June Young

Abstract

This study investigated the prevalence and subtype distribution of circulating tumor cells (CTCs) in patients with papillary thyroid cancer (PTC) before and after thyroidectomy to determine the potential of CTC count as a non-invasive marker of the efficacy of surgical treatment in PTC. Between January 2021 and January 2022, 62 PTC patients who underwent thyroidectomy at OOOOOOO Hospital were prospectively evaluated. Peripheral blood samples (7.5 ml) were collected from each patient for CTC analysis before surgery and at 2 weeks and 3 months after surgery. CTC count and the distribution of CTC subtypes, including epithelial, epithelial-mesenchymal, and mesenchymal phenotypes, were analyzed using the negative selection method and immunofluorescence staining. The relationship between CTC count and clinicopathological characteristics was analyzed before and after surgery. Before surgery, CTCs were detected in 87% (54/62) of patients; the mean CTC count was 8.0 and the median was 5.0 in 7.5 ml of peripheral blood. The mesenchymal or epithelial-mesenchymal phenotypes were predominant. After thyroidectomy, the mean and median CTC count values decreased to 5.3 and 2.5, respectively, at 2 weeks and to 4.3 and 3.0, respectively, at 3 months. This postoperative reduction in CTCs was more pronounced in patients with lymphatic invasion, lymph node metastasis, or BRAF V600E mutation. CTCs were detected in patients with PTC with a predominance of cells undergoing epithelial-mesenchymal transition. The CTC count decreased postoperatively, suggesting that liquid biopsy with CTC detection could be a valuable non-invasive tool for monitoring the efficacy of surgery in PTC.

논문정보

형식Research article
게재일2024년 03월 (BRIC 등록일 2024-03-07)
연구진국내 연구진
분야 의약학 > 종양의학

댓글 작성 로그인

CV 열람하기

연락처 보기