한빛사논문
- 조회346
Se-Yeun Hwang 1, Ji-Min Woo 1, Go Eun Choi 1, Deok-Kun Oh 2, Joo-Hyun Seo 3*, and Jin-Byung Park 1*
1Department of Food Science and Biotechnology, Ewha Womans University, Seoul 03760, Republic of Korea
2Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea
3Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Republic of Korea
*Corresponding authors: correspondence to Joo-Hyun Seo or Jin-Byung Park
Abstract
Engineering of native and recombinant enzyme reactions in whole-cell biocatalysis may allow the production of a variety of chemicals. In particular, fine-tuning of the reaction selectivity may enable the preparation of a desired product to a high conversion. Here, we demonstrated that various C9 chemicals such as 9-aminononanoic acid, 1,9-nonanediol, 9-amino-1-nonanol, and 1,9-diaminononane could be produced from renewable C18 oleic acid. As a representative example, activation of six recombinant enzyme reactions (e.g., fatty acid double bond hydratase, long-chain secondary alcohol dehydrogenase, Baeyer–Villiger monooxygenase, lipase, primary alcohol dehydrogenase, and ω-aminotransferases) with repression of one native enzyme reaction (i.e., aldehyde dehydrogenase) in Escherichia coli-based biocatalysis led to the formation of 9-aminononanoic acid with an isolation yield of 54% from oleic acid via 10-hydroxyoctadecanoic acid, 10-keto-octadecanoic acid, 9-(nonanoyloxy)nonanoic acid, 9-hydroxynonanoic acid, and 9-oxo-nonanoic acid. This study will contribute to biosynthesis of not only ω-aminoalkanoic acids but also ω-amino-1-alkanols and α,ω-diaminoalkanes from renewable fatty acids (e.g., oleic acid and ricinoleic acid).
논문정보
- Research article
- 2024년 03월 (BRIC 등록일 2024-03-05)
- 국내 연구진
- 생명과학 > 생물공학
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