한빛사논문
- 조회290
YoungWon Koo1 and GeunHyung Kim1,2,3*
1Sungkyunkwan University School of Medicine (SKKU-SOM)Suwon 16419, Republic of Korea
2Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, Republic of Korea
3Biomedical Institute for Convergence at SKKU(BICS), Sungkyunkwan University, Suwon 16419, Republic of Korea
CORRESPONDING AUTHOR : GeunHyung Kim
Abstract
In bioprinting procedures, bioinks typically comprise hydrogels, necessitating a balance between printability and bioactivity. Achieving good printability requires a relatively high hydrogel viscosity, but this can reduce bioactivity due to poor cell-to-cell interactions of the encapsulated cells. Therefore, developing hydrogels with excellent printability and bioactivity is a significant challenge in bioprinting. Here, a collagen-based bioink with excellent printability and bioactivity is developed. The bioink is prepared through a two-step process: first, by subjecting the bioink to whipping to create a non-homogeneous porous structure, and subsequently, by employing centrifugation to refine this porous structure into a uniform and relatively smaller microscale porous structure (with a specific diameter of 15.7 ± 9.2 µm). Using the designed bioink, a hierarchical porous structure is fabricated without any special equipment or supporting/sacrificing materials. To demonstrate the feasibility of the collagen bioink laden with human adipose stem cells, a hierarchical cell construct is fabricated using a bioprinter. The construct exhibits significantly enhanced cellular activities and superior osteogenic differentiation compared to the bioprinted cell constructs using normal collagen bioink. These findings suggest that collagen-based bioinks, which are both printable and bioactive, hold significant promise for extensive utilization in a variety of tissue regeneration applications.
논문정보
- Research article
- 2024년 02월 (BRIC 등록일 2024-03-04)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
