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Adv. Healthc. Mater., Mar 02 2024, | https://doi.org/10.1002/adhm.202303782 Exosome Isolation Using Chitosan Oligosaccharide Lactate-1-Pyrenecarboxylic Acid Based Self-Assembled Magnetic Nanoclusters

Minse Kim ^1,2, Chi-Yeon Song ^1,2, Jin Sil Lee ³, Yu-Rim Ahn ^1,2, Jaewon Choi ^1,2, Sang Hoon Lee ¹, SoJin Shin ^1,2, Hee Jun Na ³, Hyun-Ouk Kim ^1,2

¹Division of Chemical Engineering and Bioengineering, College of Art, Culture and Engineering, Kangwon National University, Chuncheon-si, Gangwon-do, 24341, Republic of Korea.

²Department of Smart Health Science and Technology, Kangwon National University, Chuncheon-si, Gangwon-do, 24341, Republic of Korea.

³Hauulbio, 32, Soyanggang-ro, Chuncheon-si, Gangwon-do, Republic of Korea.

M.K. and C.-Y.S. contributed equally to this work.

CORRESPONDING AUTHOR : Hyun-Ouk Kim

Abstract

Exosomes are small extracellular vesicles that play a crucial role in intercellular communication and offer significant potential for a wide range of biomedical applications. However, conventional methods for exosome isolation have limitations in terms of purity, scalability, and preservation of exosome structural integrity. To address these challenges, we present an exosome isolation platform using chitosan oligosaccharide lactate conjugated 1-pyrenecarboxylic acid (COL-Py) based self-assembled magnetic nanoclusters (CMNCs). We characterized CMNCs to optimize their size, stability, and interaction dynamics with exosomes. The efficiency of CMNCs in isolating exosomes was systematically evaluated using various analytical methods to demonstrate their ability to capture exosomes based on amphiphilic lipid bilayers.CMNC-based exosome isolation consistently yielded exosomes with structural integrity and purity similar to those obtained using traditional methods. The reusability of CMNCs over multiple exosome isolation cycles underscores their scalability and offers an efficient solution for biomedical applications. These results were supported by western blot analysis, which demonstrated the superiority of CMNC-based isolation in terms of purity compared to conventional methods.By providing a scalable and efficient exosome isolation process that preserves both structural integrity and purity, CMNCs could constitute a new platform that can contribute to the field of exosome studies. This article is protected by copyright. All rights reserved.

논문정보

형식Research article
게재일2024년 03월 (BRIC 등록일 2024-03-04)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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