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Trends Biotechnol., Feb 28 2024, S0167-7799(24)00037-4 | https://doi.org/10.1016/j.tibtech.2024.02.008 Reduction-to-synthesis: the dominant approach to genome-scale synthetic biology

Kangsan Kim ^1,2,7, Donghui Choe ^3,7, Suhyung Cho 2, Bernhard Palsson ^3,4, Byung-Kwan Cho ^1,2,5,6

¹Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea

²KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea

³Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA

⁴Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Kongens, Lyngby, Denmark

⁵Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea

⁶http://cholab.or.kr/

⁷These authors contributed equally to this work.

Correspondence: Byung-Kwan Cho

Abstract

Advances in systems and synthetic biology have propelled the construction of reduced bacterial genomes. Genome reduction was initially focused on exploring properties of minimal genomes, but more recently it has been deployed as an engineering strategy to enhance strain performance. This review provides the latest updates on reduced genomes, focusing on dual-track approaches of top-down reduction and bottom-up synthesis for their construction. Using cases from studies that are based on established industrial workhorse strains, we discuss the construction of a series of synthetic phenotypes that are candidates for biotechnological applications. Finally, we address the possible uses of reduced genomes for biotechnological applications and the needed future research directions that may ultimately lead to the total synthesis of rationally designed genomes.

논문정보

형식Review Paper
게재일2024년 02월 (BRIC 등록일 2024-03-05)
연구진국내(교신)+국외 연구진
분야 생명과학 > 생물공학

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