김태진 (성균관대학교 의과대학)

한빛사논문

조회357

성균관대학교 의과대학

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Cell. Mol. Immunol., Mar 01 2024, | https://doi.org/10.1038/s41423-024-01126-0 Two distinct subpopulations of marginal zone B cells exhibit differential antibody-producing capacities and radioresistance

Sujin Lee ¹, Yeunjung Ko ^1,3, Hyun Woo Lee ¹, Won Joon Oh ¹, Hun Gi Hong ¹, Dinuka Ariyaratne ², Se Jin Im ^1,* and Tae Jin Kim ^1,*

¹Department of Immunology, Graduate School of Basic Medical Science, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea.
²Department of Immunology and Molecular Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
³Present address: Immunology and Microbiology Graduate Program, Baylor College of Medicine, Houston, TX 77030, USA.

^*Corresponding authors: correspondence to Se Jin Im or Tae Jin Kim

Abstract

Marginal zone (MZ) B cells, which are splenic innate-like B cells that rapidly secrete antibodies (Abs) against blood-borne pathogens, are composed of heterogeneous subpopulations. Here, we showed that MZ B cells can be divided into two distinct subpopulations according to their CD80 expression levels. CD80^high MZ B cells exhibited greater Ab-producing, proliferative, and IL-10-secreting capacities than did CD80^low MZ B cells. Notably, CD80^high MZ B cells survived 2-Gy whole-body irradiation, whereas CD80^low MZ B cells were depleted by irradiation and then repleted with one month after irradiation. Depletion of CD80^low MZ B cells led to accelerated development of type II collagen (CII)-induced arthritis upon immunization with bovine CII. CD80^high MZ B cells exhibited higher expression of genes involved in proliferation, plasma cell differentiation, and the antioxidant response. CD80^high MZ B cells expressed more autoreactive B cell receptors (BCRs) that recognized double-stranded DNA or CII, expressed more immunoglobulin heavy chain sequences with shorter complementarity-determining region 3 sequences, and included more clonotypes with no N-nucleotides or with B-1a BCR sequences than CD80^low MZ B cells. Adoptive transfer experiments showed that CD21⁺CD23⁺ transitional 2 MZ precursors preferentially generated CD80^low MZ B cells and that a proportion of CD80^low MZ B cells were converted into CD80^high MZ B cells; in contrast, CD80^high MZ B cells stably remained CD80^high MZ B cells. In summary, MZ B cells can be divided into two subpopulations according to their CD80 expression levels, Ab-producing capacity, radioresistance, and autoreactivity, and these findings may suggest a hierarchical composition of MZ B cells with differential stability and BCR specificity.

논문정보

형식Research article
게재일2024년 03월 (BRIC 등록일 2024-03-05)
연구진국내(교신)+국외 연구진
분야 의약학 > 면역의학

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