Thuy Thi Le (기초과학연구원, 성균관대학교)

한빛사논문

조회201

Thuy Thi LeThuy Thi Le

기초과학연구원, 성균관대학교

Sci. Adv., Mar 2024, 10 (9) | https://doi.org/10.1126/sciadv.adm7605 Mapping cerebral perfusion in mice under various anesthesia levels using highly sensitive BOLD MRI with transient hypoxia

Thuy Thi Le^1,2,3, Geun Ho Im¹, Chan Hee Lee¹, Sang Han Choi¹, Seong-Gi Kim^1,2,3*

¹center for neuroscience imaging Research (cniR), institute for Basic Science (iBS), Suwon, Republic of Korea.

²department of Biomedical engineering, Sungkyunkwan university, Suwon, Republic of Korea.

³department of intelligent Precision health-care convergence, Sungkyunkwan university, Suwon, Republic of Korea.

*corresponding author : Seong-Gi Kim

Abstract

Cerebral perfusion is critical for the early detection of neurological diseases and for effectively monitoring disease progression and treatment responses. Mouse models are widely used in brain research, often under anesthesia, which can affect vascular physiology. However, the impact of anesthesia on regional cerebral blood volume and flow in mice has not been thoroughly investigated. In this study, we have developed a whole-brain perfusion MRI approach by using a 5-second nitrogen gas stimulus under inhalational anesthetics to induce transient BOLD dynamic susceptibility contrast (DSC). This method proved to be highly sensitive, repeatable within each imaging session, and across four weekly sessions. Relative cerebral blood volumes measured by BOLD DSC agree well with those by contrast agents. Quantitative cerebral blood volume and flow metrics were successfully measured in mice under dexmedetomidine and various isoflurane doses using both total vasculature-sensitive gradient-echo and microvasculature-sensitive spin-echo BOLD MRI. Dexmedetomidine reduces cerebral perfusion, while isoflurane increases cerebral perfusion in a dose-dependent manner.

논문정보

형식Research article
게재일2024년 03월 (BRIC 등록일 2024-02-29)
연구진국내 연구진
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