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김성은Seongeun Kim

KAIST

Adv. Healthc. Mater., Feb 27 2024, | https://doi.org/10.1002/adhm.202303272 Nanotopology-Enabled On-Site Pathogen Detection for Managing Atopic Dermatitis

Seongeun Kim ^{1^†}, Younseong Song^{1^†}, Jueun Kim ^{2^†}, Booseok Jeong ¹, Nahyun Park ¹, Yoo Min Park ³, Yong Tae Kim ⁴, Donggee Rho ³, Seok Jae Lee ³, Bong Gill Choi², Sung Gap Im ¹, Kyoung G. Lee³

¹Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.

²Department of Chemical Engineering, Kangwon National University, Samcheok, 25913, Republic of Korea.

³Nanobio Application Team, National NanoFab Center, Daejeon, 34141, Republic of Korea.

⁴Department of Chemical Engineering & Biotechnology, Tech University of Korea, Siheung-si, 15073, Republic of Korea.

^†These authors contributed equally.

CORRESPONDING AUTHORS : Bong Gill Choi, Sung Gap Im, Kyoung G. Lee

Abstract

Atopic dermatitis (AD), a prevalent skin condition often complicated by microbial infection, poses a significant challenge in identifying the responsible pathogen for its effective management. However, a reliable, safe tool for pinpointing the source of these infections remains elusive. In this study, we propose a novel on-site pathogen detection that combines chemically functionalized nanotopology with genetic analysis to capture and analyze pathogens closely associated with severe atopic dermatitis. The chemically functionalized nanotopology features a 3D hierarchical nanopillar array (HNA) with a functional polymer coating, tailored to isolate target pathogens from infected skin. This innovative nanotopology demonstrates superior pathogenic capture efficiency, favorable entrapment patterns, and non-cytotoxicity. We utilize an HNA-assembled stick to directly retrieve bacteria from infected skin samples, followed by extraction-free quantitative loop-mediated isothermal amplification (direct qLAMP) for validation. To mimic human skin conditions, we employ porcine skin, successfully capturing Staphylococcus aureus, a common bacterium exacerbating AD cases. Our on-site detection method exhibits an impressive detection limit of 10^3 cells/mL. The HNA-assembled stick represents a promising tool for on-site detection of bacteria associated with atopic dermatitis. This innovative approach enables to deepen our understanding of AD pathogenesis and open avenues for more effective management strategies for chronic skin condition. This article is protected by copyright. All rights reserved.

논문정보

형식Research article
게재일2024년 02월 (BRIC 등록일 2024-03-11)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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