김동현 (경희대학교)

한빛사논문

조회243

경희대학교

Gut Microbes, 2024, 16 (1) | https://doi.org/10.1080/19490976.2024.2319844 Regulation of colonic neuropeptide Y expression by the gut microbiome in patients with ulcerative colitis and its association with anxiety- and depression-like behavior in mice

Min-Kyung Joo ^{1 2 †}, Jae-Won Lee ^{1 2 †}, Jeong-Hwa Woo², Hyo-Jong Kim ³, Dong-Hyun Kim ^{1 2 *}, Jung-Hye Choi ^{1 2 *}

¹Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul, Korea.

²College of Pharmacy, Kyung Hee University, Seoul, Korea.

³Department of Internal Medicine, School of Medicine, Kyung Hee University, Seoul, Korea.

^†These authors contributed equally to this work
^*Corresponding authors: correspondence to Dong-Hyun Kim or Jung-Hye Choi

Abstract

Patients with inflammatory bowel disease (IBD), including ulcerative colitis (UC), show an increased incidence of anxiety and depression; however, the association between UC-associated psychiatric disorders and the gut microbiota is unclear. This study aimed to examine whether gut microbiota from patients with UC can alter colonic gene expression, leading to anxiety- and depression-like behavior in mice receiving fecal microbiota transplantation (FMT). RNA sequencing transcriptome analyses revealed a difference in colonic gene expression between mice receiving FMT from patients with UC (UC-FMT mice) and those receiving FMT from healthy controls (HC-FMT mice). Gene ontology analysis revealed the downregulation of neuropeptide signaling pathways, including neuropeptide Y (NPY) expression, in the colons of UC-FMT mice. The protein levels of NPY also decreased in the colon and plasma of UC-FMT mice compared to those in HC-FMT mice. The oral administration of Enterococcus mundtii (EM), a bacterium isolated from the feces of patients with UC, reduced NPY expression in the colons of mice and induced intestinal inflammation, anxiety, and depression-like behavior. Reduced NPY protein levels were also observed in the plasma and hippocampus of EM-treated mice. Intraperitoneal administration of NPY significantly alleviated anxiety- and depressive-like behaviors induced by EM in mice. Capsular polysaccharide in EM was associated with EM-induced NPY downregulation in the colon. Analysis of Gene Expression Omnibus datasets showed markedly reduced NPY expression in the inflamed colons of patients with UC compared with that in the colons of healthy controls. In summary, EM-induced reduction in the colonic expression of NPY may be associated with a decrease in hippocampal NPY and anxiety- and depression-like behavior in mice.

논문정보

형식Research article
게재일2024년 02월 (BRIC 등록일 2024-02-27)
연구진국내 연구진
분야 생명과학 > 노화/암생물학

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