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한빛사논문

조회184

순천향대학교

Biomaterials, Feb 19 2024, 307 S0142-9612(24)00042-5 | https://doi.org/10.1016/j.biomaterials.2024.122508 Multi-functional dual-layer nanofibrous membrane for prevention of postoperative pancreatic leakage

Prayas Chakma Shanto^a, Md Abdullah Al Fahad ^a, Hae Il Jung ^b,c, Myeongki Park ^a, Hyeyoung Kim ^b,c, Sang Ho Bae ^b,c, Byong-Taek Lee ^a,b

^aDepartment of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea

^bInstitute of Tissue Regeneration, Soonchunhyang University, Cheonan, Republic of Korea

^cDepartment of General Surgery, Soonchunhyang University Hospital, Cheonan, Republic of Korea

Corresponding authors: Hae Il Jung, Byong-Taek Lee

Abstract

Postoperative pancreatic leakage due to pancreatitis in patients is a life-threatening surgical complication. The majority of commercial barriers are unable to meet the demands for pancreatic leakage due to poor adhesiveness, toxicity, and inability to degrade. In this study, we fabricated mitomycin-c and thrombin-loaded multifunctional dual-layer nanofibrous membrane with a combination of alginate, PCL, and gelatin to resolve the leakage due to suture line disruption, promote hemostasis, wound healing, and prevent postoperative tissue adhesion. Electrospinning was used to fabricate the dual-layer system. The study results demonstrated that high gelatin and alginate content in the inner layer decreased the fiber diameter and water contact angle, and crosslinking allowed the membrane to be more hydrophilic, making it highly biodegradable, and adhering firmly to the tissue surfaces. The results of in vitro biocompatibility and hemostatic assay revealed that the dual-layer had a higher cell proliferation and showed effective hemostatic properties. Moreover, the in vivo studies and in silico molecular simulation indicated that the dual layer was covered at the wound site, prevented suture disruption and leakage, inhibited hemorrhage, and reduced postoperative tissue adhesion. Finally, the study results proved that dual-layer multifunctional nanofibrous membrane has a promising therapeutic potential in preventing postoperative pancreatic leakage.

논문정보

형식Research article
게재일2024년 02월 (BRIC 등록일 2024-03-06)
연구진국내 연구진
분야 의약학 > 재생의학

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