한빛사논문
- 조회325
Euiyeon Lee1,2, Hye Kyu Choi1, Youngeun Kwon2, Ki-Bum Lee2
1Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854 USA
2Department of Biomedical Engineering, Dongguk University, Seoul, 04620 South Korea
CORRESPONDING AUTHORS : Youngeun Kwon, Ki-Bum Lee
Abstract
Real-time and non-invasive monitoring of neuronal differentiation helps to increase understanding of neuronal development and develop stem cell therapies for neurodegenerative diseases. Conventional methods such as RT-PCR, western blotting, and immunofluorescence (IF), lack single-cell-level resolution and require invasive procedures, fixation, and staining. These limitations hinder accurate monitoring progress of neural stem cell (NSC) differentiation and understanding its functions. Herein, a novel approach is reported to non-invasively monitor neuronal differentiation in real-time using cell-based biosensors (CBBs) that detects hippocalcin, biomarker of neuronal differentiation. To construct the hippocalcin sensor proteins, two different hippocalcin bioreceptors are fused to each split-intein, carrying split-nuclear localization signal (NLS) peptides, respectively, and fluorescent protein is introduced as reporter. CBBs operated in the presence of hippocalcin to generate functional signal peptides, which promptly translocated the fluorescence signal to the nucleus. The NSC-based biosensor shows fluorescence signal translocation only upon neuronal differentiation and not undifferentiated stem cells or glial cells. Furthermore, this approach allows monitoring of neural differentiation at earlier stages than detected using IF staining. It is believed that novel CBBs offer an alternative to current techniques by capturing the dynamics of differentiation progress at the single-cell-level and providing a tool to evaluate how NSCs efficiently differentiate into neurons.
논문정보
- Research article
- 2024년 02월 (BRIC 등록일 2024-02-26)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
