한빛사
>
한빛사논문
한빛사논문
- 조회258
Proc. Natl. Acad. Sci. USA, Feb 27 2024, 121 (9) | https://doi.org/10.1073/pnas.2314620121
Lutetium texaphyrin: A photocatalyst that triggers pyroptosis via biomolecular photoredox catalysis
Yunjie Xua, Calvin V. Chaub, Jieun Leea, Adam C. Sedgwickb, Le Yua, Mingle Lic,1, Xiaojun Pengc,1, Jong Seung Kima,d,1, and Jonathan L. Sesslerb,1
aDepartment of Chemistry, Korea University, Seoul 02841, Korea;
bDepartment of Chemistry, The University of Texas at Austin, Austin, TX 78712- 1224;
cCollege of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China; and
dTheranoChem Incorporation, Seongbuk- gu, Seoul 02856, Korea
1To whom correspondence may be addressed. : Mingle Li, Xiaojun Peng, Jong Seung Kim, and Jonathan L. Sessler
Abstract
Photon-controlled pyroptosis activation (PhotoPyro) is a promising technique for cancer immunotherapy due to its noninvasive nature, precise control, and ease of operation. Here, we report that biomolecular photoredox catalysis in cells might be an important mechanism underlying PhotoPyro. Our findings reveal that the photocatalyst lutetium texaphyrin (
MLu) facilitates rapid and direct photoredox oxidation of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and various amino acids, thereby triggering pyroptosis through the caspase 3/GSDME pathway. This mechanism is distinct from the well-established role of
MLu as a photodynamic therapy sensitizer in cells. Two analogs of
MLu, bearing different coordinated central metal cations, were also explored as controls. The first control, gadolinium texaphyrin (
MGd), is a weak photocatalyst but generates reactive oxygen species (ROS) efficiently. The second control, manganese texaphyrin (
MMn), is ineffective as both a photocatalyst and a ROS generator. Neither
MGd nor
MMn was found to trigger pyroptosis under the conditions where
MLu was active. Even in the presence of a ROS scavenger, treating MDA-MB-231 cells with
MLu at concentrations as low as 50 nM still allows for pyroptosis photo-activation. The present findings highlight how biomolecular photoredox catalysis could contribute to pyroptosis activation by mechanisms largely independent of ROS.
논문정보
- Research article
- 2024년 02월 (BRIC 등록일 2024-02-23)
- 국내(교신)+국외 연구진
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
