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Int. J. Surg., Feb 21 2024, | https://doi.org/10.1097/JS9.0000000000001227 Detection of metastatic lymph node and sentinel lymph node mapping using mannose receptor targeting in in vivo mouse and rabbit uterine cancer models

Jeong, Sohyeon^a; Jeon, Ok Hwa^b,c; Hong, Jin Hwa^a; Kim, Kyungsu^b,c; Kim, Beop-Min^d; Park, Ji Yong^e; Kim, Kweon^f; Cho, Hyun-Woong^a; Kim, Hyun Koo^b,c

^aDepartment of Obstetrics and Gynecology, Korea University Guro Hospital, Seoul 08308, Republic of Korea

^bDepartment of Thoracic and Cardiovascular Surgery, Korea University Guro Hospital, Korea University College of Medicine, Seoul 08308, Republic of Korea

^cDepartment of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Republic of Korea

^dDepartment of Bio-Convergence, Korea University, Seoul 02841, Republic of Korea

^eDepartment of Nuclear Medicine, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea

^fCellbion Co., Ltd., Seoul 03127, Republic of Korea

Sohyeon Jeong and Ok Hwa Jeon: S.J. and O.H.J. contributed equally to this work.

^*Corresponding authors: H K. Kim, H-W. Cho

Abstract

This study aimed to evaluate the effectiveness of neo-mannosyl human serum albumin-indocyanine green (MSA-ICG) for detecting metastatic lymph node and mapping sentinel lymph node (SLN) using mouse footpad uterine tumor models. Additionally, we assessed the feasibility of MSA-ICG in SLN mapping in rabbit uterine cancer models. We compared the LN targeting ability of MSA-ICG with ICG. Six mouse footpad tumor models and two normal mice were each assigned to MSA-ICG and ICG, respectively. After the assigned tracers were injected, fluorescence images were taken, and we compared the signal-to-background ratio (SBR) of the tracers. A SLN biopsy was performed to confirm LN metastasis status and CD206 expression level. Finally, an intraoperative SLN biopsy was performed in rabbit uterine cancer models using MSA-ICG. We detected 14 groin LNs out of 16 in the MSA-ICG and ICG groups. The SBR of the MSA-ICG group was significantly higher than that of the ICG group. The metastatic LN subgroup of MSA-ICG showed a significantly higher SBR than that of ICG. CD206 was expressed at a high level in metastatic LN, and the signal intensity difference increased as the CD206 expression level increased. SLN mapping was successfully performed in two of the three rabbit uterine cancer models. MSA-ICG was able to distinguish metastatic LN for an extended period due to its specific tumor-associated macrophage-targeting property. Therefore, it may be a more distinguishable tracer for identifying metastatic LNs and SLNs during uterine cancer surgery. Further research is needed to confirm these results.

논문정보

형식Research article
게재일2024년 02월 (BRIC 등록일 2024-02-21)
연구진국내 연구진
분야 의약학 > 종양의학

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