전성윤 (KAIST)

한빛사논문

조회338

전성윤Jessie S. Jeon

KAIST

CV File 188 kb

Bioact. Mater., Available online 8 January 2024 | https://doi.org/10.1016/j.bioactmat.2023.12.021 Hydrophobic surface induced pro-metastatic cancer cells for in vitro extravasation models

Minseok Lee ^a,1, Seunggyu Kim ^b,1, Sun Young Lee ^c, Jin Gyeong Son ^c, Joonha Park ^b, Seonghyeon Park ^a, Jemin Yeun ^a, Tae Geol Lee ^c, Sung Gap Im ^a,d, Jessie S. Jeon^b

^aDepartment of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daehak-ro 291, Yuseong-gu, Daejeon, 34141, Republic of Korea

^bDepartment of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daehak-ro 291, Yuseong-gu, Daejeon, 34141, Republic of Korea

^cBioimaging Team, Safety Measurement Institute, Korea Research Institute of Standards and Science (KRISS), Gajeong-ro 267, Yuseong-gu, Daejeon, 34113, Republic of Korea

^dKAIST Institute for the NanoCentury (KINC), Korea Advanced Institute of Science and Technology, Daehak-ro 291, Yuseong-gu, Daejeon, 34141, Republic of Korea

¹These authors contributed equally.

Corresponding authors: Tae Geol Lee, Sung Gap Im, Jessie S. Jeon

Abstract

In vitro vascularized cancer models utilizing microfluidics have emerged as a promising tool for mechanism study and drug screening. However, the lack of consideration and preparation methods for cancer cellular sources that are capable of adequately replicating the metastatic features of circulating tumor cells contributed to low relevancy with in vivo experimental results. Here, we show that the properties of cancer cellular sources have a considerable impact on the validity of the in vitro metastasis model. Notably, with a hydrophobic surface, we can create highly metastatic spheroids equipped with aggressive invasion, endothelium adhesion capabilities, and activated metabolic features. Combining these metastatic spheroids with the well-constructed microfluidic-based extravasation model, we validate that these metastatic spheroids exhibited a distinct extravasation response to epidermal growth factor (EGF) and normal human lung fibroblasts compared to the 2D cultured cancer cells, which is consistent with the previously reported results of in vivo experiments. Furthermore, the applicability of the developed model as a therapeutic screening platform for cancer extravasation is validated through profiling and inhibition of cytokines. We believe this model incorporating hydrophobic surface-cultured 3D cancer cells provides reliable experimental data in a clear and concise manner, bridging the gap between the conventional in vitro models and in vivo experiments.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-02-15)
연구진국내 연구진
분야 바이오·의료융합 > 의료기기

댓글 작성 로그인

CV 열람하기

연락처 보기