전은영 (한양대학교)

한빛사논문

조회425

한양대학교

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Nat. Commun., Feb 14 2024, 15 (1) | https://doi.org/10.1038/s41467-024-45467-w Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection

Eunyoung Jeon ^1,2,3,9, Bonhan Koo ^4,9, Suyeon Kim ^1,2,3,9, Jieun Kim ⁵, Yeonuk Yu ⁵, Hyowon Jang⁶, Minju Lee ⁴, Sung-Han Kim ⁷, Taejoon Kang ⁶, Sang Kyung Kim ⁸, Rhokyun Kwak ^5,*, Yong Shin ^4,* & Joonseok Lee ^1,2,3,*

¹Department of Chemistry, Hanyang University, Seoul 04763, Republic of Korea.
²Research Institute for Natural Science, Hanyang University, Seoul 04763, Republic of Korea.
³Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul 04763, Republic of Korea.
⁴Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
⁵Department of Mechanical Convergence Engineering, Hanyang University, Seoul 04763, Republic of Korea.
⁶Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
⁷Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea.
⁸Center for Augmented Safety Systems with Intelligence, Sensing and Tracking (ASSIST), Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.
⁹These authors contributed equally: Eunyoung Jeon, Bonhan Koo, Suyeon Kim.

^*Corresponding authors: correspondence to Rhokyun Kwak, Yong Shin or Joonseok Lee

Abstract

Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids.

논문정보

형식Research article
게재일2024년 02월 (BRIC 등록일 2024-02-15)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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