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Nucleic Acids Res., Feb 10 2024, gkae083 | https://doi.org/10.1093/nar/gkae083 RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination

Jeong H. Joo^1,†, Soogil Hong^1,†, Mika T. Higashide^2,3, Eui-Hwan Choi^1,4, Seobin Yoon¹, Min-Su Lee¹, Hyun Ah Kang¹, Akira Shinohara², Nancy Kleckner⁵ and Keun P. Kim^1,*

¹Department of Life Sciences, Chung-Ang University, Seoul 06974, South Korea

²Institute for Protein Research, Graduate School of Science, Osaka University, Osaka 565-0871, Japan

³Current address: Graduate School of Engineering, Fukui University, Fukui 910-8507, Japan

⁴New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Deagu 41061, South Korea

⁵Department of Molecular and Cellular Biology, Harvard University, Cambridge 02138, USA

*To whom correspondence should be addressed. : Keun P. Kim

†The first two authors should be regarded as Joint First Authors.

Abstract

Meiotic recombination is initiated by programmed double-strand breaks (DSBs). Studies in Saccharomyces cerevisiae have shown that, following rapid resection to generate 3' single-stranded DNA (ssDNA) tails, one DSB end engages a homolog partner chromatid and is extended by DNA synthesis, whereas the other end remains associated with its sister. Then, after regulated differentiation into crossover- and noncrossover-fated types, the second DSB end participates in the reaction by strand annealing with the extended first end, along both pathways. This second-end capture is dependent on Rad52, presumably via its known capacity to anneal two ssDNAs. Here, using physical analysis of DNA recombination, we demonstrate that this process is dependent on direct interaction of Rad52 with the ssDNA binding protein, replication protein A (RPA). Furthermore, the absence of this Rad52-RPA joint activity results in a cytologically-prominent RPA spike, which emerges from the homolog axes at sites of crossovers during the pachytene stage of the meiotic prophase. Our findings suggest that this spike represents the DSB end of a broken chromatid caused by either the displaced leading DSB end or the second DSB end, which has been unable to engage with the partner homolog-associated ssDNA. These and other results imply a close correspondence between Rad52-RPA roles in meiotic recombination and mitotic DSB repair.

논문정보

형식Research article
게재일2024년 02월 (BRIC 등록일 2024-02-14)
연구진국내(교신)+국외 연구진
분야 생명과학 > 유전학

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