박혜상 (Massachusetts General Hospital and Harvard Medical School, 한림대학교 의과대학)

한빛사논문

조회288

Massachusetts General Hospital and Harvard Medical School, 한림대학교 의과대학

CV File 620 kb

Biomater. Res., 2024 Jan 25:28:0002 | https://doi.org/10.34133/bmr.0002 Bifunctional Tumor-Targeted Bioprobe for Phothotheranosis

Hae Sang Park ^1,2, Shinya Yokomizo ¹, Haoran Wang ¹, Sophia Manganiello¹, Hailey Monaco ¹, Rose McDonnell ¹, Hajin Joanne Kim ¹, Jiyun Rho ¹, Sung Ahn ¹, Harry Jung ³, Homan Kang¹, Kai Bao ¹, Satoshi Kashiwagi ^1*, Hak Soo Choi^1*

¹Gordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.

²Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Hallym University, Chuncheon 24253, South Korea.

³Institute of New Frontier Research Team, Hallym Clinical and Translation Science Institute, Hallym University, Chuncheon 24252, South Korea.

*Address correspondence to: H.S.C.; S.K.

Abstract

Background: Near-infrared (NIR) phototheranostics provide promising noninvasive imaging and treatment for head and neck squamous cell carcinoma (HNSCC), capitalizing on its adjacency to skin or mucosal surfaces. Activated by laser irradiation, targeted NIR fluorophores can selectively eradicate cancer cells, harnessing the power of synergistic photodynamic therapy and photothermal therapy. However, there is a paucity of NIR bioprobes showing tumor-specific targeting and effective phototheranosis without hurting surrounding healthy tissues. Methods: We engineered a tumor-specific bifunctional NIR bioprobe designed to precisely target HNSCC and induce phototheranosis using bioconjugation of a cyclic arginine-glycine-aspartic acid (cRGD) motif and zwitterionic polymethine NIR fluorophore. The cytotoxic effects of cRGD-ZW800-PEG were measured by assessing heat and reactive oxygen species (ROS) generation upon an 808-nm laser irradiation. We then determined the in vivo efficacy of cRGD-ZW800-PEG in the FaDu xenograft mouse model of HNSCC, as well as its biodistribution and clearance, using a customized portable NIR imaging system. Results: Real-time NIR imaging revealed that intravenously administered cRGD-ZW800-PEG targeted tumors rapidly within 4 h postintravenous injection in tumor-bearing mice. Upon laser irradiation, cRGD-ZW800-PEG produced ROS and heat simultaneously and exhibited synergistic photothermal and photodynamic effects on the tumoral tissue without affecting the neighboring healthy tissues. Importantly, all unbound bioprobes were cleared through renal excretion. Conclusions: By harnessing phototheranosis in combination with tailored tumor selectivity, our targeted bioprobe ushers in a promising paradigm in cancer treatment. It promises safer and more efficacious therapeutic avenues against cancer, marking a substantial advancement in the field.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-02-13)
연구진국내+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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