이정찬 (Harvard University, KAIST)

한빛사논문

조회261

이정찬Jeong-Chan Lee

Harvard University, KAIST

Nat. Commun., Feb 08 2024, 15 (1) 711 | https://doi.org/10.1038/s41467-024-44822-1 Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties

Jeong-Chan Lee^1,2,11, Su Yeong Kim^2,11, Jayeon Song^3,4,5,11, Hyowon Jang³, Min Kim², Hanul Kim⁶, Siyoung Q. Choi⁶, Sunjoo Kim⁷, Pawan Jolly¹, Taejoon Kang^3,8, Steve Park² & Donald E. Ingber^1,9,10

¹Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02215, USA.

²Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.

³Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.

⁴Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA 02114, USA.

⁵Department of Radiology, Harvard Medical School, Boston, MA 02114, USA.

⁶Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 34141, Republic of Korea.

⁷Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju-si, Gyeongsangnam-do 52727, Republic of Korea.

⁸School of Pharmacy, Sungkyunkwan University (SKKU), Suwon-si, Gyeongido 16419, Republic of Korea.

⁹Vascular Biology Program and Department of Surgery, Boston Children’s Hospital and Harvard Medical School, Boston, MA 02115, USA.

¹⁰Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

¹¹These authors contributed equally: Jeong-Chan Lee, Su Yeong Kim, Jayeon Song.

Correspondence and requests for materials should be addressed to Taejoon Kang, Steve Park or Donald E. Ingber.

Abstract

Development of coating technologies for electrochemical sensors that consistently exhibit antifouling activities in diverse and complex biological environments over extended time is vital for effective medical devices and diagnostics. Here, we describe a micrometer-thick, porous nanocomposite coating with both antifouling and electroconducting properties that enhances the sensitivity of electrochemical sensors. Nozzle printing of oil-in-water emulsion is used to create a 1 micrometer thick coating composed of cross-linked albumin with interconnected pores and gold nanowires. The layer resists biofouling and maintains rapid electron transfer kinetics for over one month when exposed directly to complex biological fluids, including serum and nasopharyngeal secretions. Compared to a thinner (nanometer thick) antifouling coating made with drop casting or a spin coating of the same thickness, the thick porous nanocomposite sensor exhibits sensitivities that are enhanced by 3.75- to 17-fold when three different target biomolecules are tested. As a result, emulsion-coated, multiplexed electrochemical sensors can carry out simultaneous detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid, antigen, and host antibody in clinical specimens with high sensitivity and specificity. This thick porous emulsion coating technology holds promise in addressing hurdles currently restricting the application of electrochemical sensors for point-of-care diagnostics, implantable devices, and other healthcare monitoring systems.

논문정보

형식Research article
게재일2024년 02월 (BRIC 등록일 2024-02-13)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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