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고려대학교

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Bioresour. Technol., Feb 03 2024, S0960-8524(24)00119-6 | https://doi.org/10.1016/j.biortech.2024.130416 Isopropanol production using engineered corynebacterium glutamicum from waste rice straw biomass

Xiaoyu Shi ^a, Joonhee Chang ^a, Minhye Kim^a, Myeong-Eun Lee ^a,b, Ha-Young Shin ^a, Sung Ok Han ^a,b

^aDepartment of Biotechnology, Korea University, Seoul 02841, Republic of Korea

^bInstitute of Life Science and Natural Resources, Korea University, Seoul 02841, Republic of Korea

Corresponding author : Sung Ok Han

Abstract

Isopropanol, a well-known biofuel, is a widely used precursor for chemical products that can replace nonrenewable petroleum energy. Here, engineered Corynebacterium glutamicum that can effectively utilize all xylose and glucose in agricultural waste rice straw to produce isopropanol was described. First, codon mutations were introduced into transporters and glycolytic-related genes to decrease the glucose preference of C. glutamicum. A more energetically favorable xylose oxidative pathway was constructed that replaced traditional xylose isomerization pathways, saving twice the number of enzymatic steps. A succinate auxiliary module was incorporated into the tricarboxylic acid cycle (TCA), connecting the xylose-utilized pathway with the isopropanol pathway to maximize xylose orientation towards the product. The final engineered strain successfully consumed 100 % of the xylose from NaOH-pretreated, enzyme-hydrolyzed rice straw and effectively synthesized 4.91 g/L isopropanol. This study showcases the successful conversion of agricultural waste into renewable energy, unveiling new possibilities for advancing biological fermentation technology.

논문정보

형식Research article
게재일2024년 02월 (BRIC 등록일 2024-02-07)
연구진국내 연구진
분야 생명과학 > 생물공학

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