한빛사논문
- 조회394
Dohee Koo 1,†, Hong Gil Lee 2,†, Soon Hyung Bae 1, Kyounghee Lee 3, and Pil Joon Seo 1,2,3,*
1Department of Chemistry, Seoul National University, Seoul 08826, Korea
2Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea
3Research Institute of Basic Sciences, Seoul National University, Seoul 08826, Korea
†These authors contributed equally to this work.
*Corresponding author: correspondence to Pil Joon Seo
Abstract
Plants are aerobic organisms that rely on molecular oxygen for respiratory energy production. Hypoxic conditions, with oxygen levels ranging between 1% and 5%, usually limit aerobic respiration and affect plant growth and development. Here, we demonstrate that hypoxic microenvironment induced by active cell proliferation during the two-step plant regeneration process intrinsically represses the regeneration competence of callus in Arabidopsis thaliana. Hypoxia-repressed plant regeneration was mediated by the RELATED TO APETALA 2.12 (RAP2.12) protein, a member of the Ethylene Response Factor VII (ERF-VII) family. The hypoxia-activated RAP2.12 protein promoted salicylic acid (SA) biosynthesis and defense responses, inhibiting pluripotency acquisition and de novo shoot regeneration in calli. RAP2.12 could bind directly to the SALICYLIC ACID INDUCTION DEFICIENT 2 (SID2) gene promoter and activate SA biosynthesis, repressing plant regeneration via a PLETHORA (PLT)-dependent pathway. The rap2.12 mutant calli exhibited enhanced shoot regeneration, which was impaired by SA treatment. Taken together, our findings demonstrate that cell proliferation-dependent hypoxic microenvironment reduces cellular pluripotency and plant regeneration through the RAP2.12–SID2 module.
논문정보
- Research article
- 2024년 01월 (BRIC 등록일 2024-02-02)
- 국내 연구진
- 생명과학 > 식물학
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