박현규 (KAIST) | 한빛사논문 > 한빛사

한빛사논문

조회372

KAIST

CV File 68 kb

Biosens. Bioelectron., Jan 10 2024, 250 S0956-5663(24)00022-8 | https://doi.org/10.1016/j.bios.2024.116019 A label-free and washing-free method to detect biological thiols on a personal glucose meter utilizing glucose oxidase-mimicking activity of gold nanoparticles

Sang Mo Lee, Hyoyong Kim, Pei Li, Hyun Gyu Park

Department of Chemical and Biomolecular Engineering (BK21 Four), Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea

Corresponding author : Hyun Gyu Park

Abstract

We herein developed a label-free and washing-free method to detect biological thiols (biothiols) on a personal glucose meter (PGM) utilizing the intrinsic glucose oxidase (GOx)-mimicking activity of gold nanoparticles (AuNPs). By focusing on the fact that this activity could be diminished by target biothiols through their binding onto the AuNP surface, we correlated the concentration of biothiols with that of glucose readily measurable on a PGM and successfully determined cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) down to 0.116, 0.059, and 0.133 μM, respectively, with high specificity against non-target biomolecules. We further demonstrated its practical applicability by reliably detecting target biothiol in heterogeneous human serum. Due to the meritorious features of PGM such as simplicity, portability, and cost-effectiveness, we believe that this work could serve as a powerful platform for biothiol detection in point-of-care settings.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-02-05)
연구진국내 연구진
분야 생명과학 > 생물공학

댓글 작성 로그인

CV 열람하기

연락처 보기