전용근 (서울대학교) | 한빛사논문 > 한빛사

한빛사논문

조회797

서울대학교

Nat. Commun., Jan 31 2024, 15 (1) | https://doi.org/10.1038/s41467-024-45270-7 Ubiquitin ligase RNF20 coordinates sequential adipose thermogenesis with brown and beige fat-specific substrates

Yong Geun Jeon¹, Hahn Nahmgoong¹, Jiyoung Oh², Dabin Lee³, Dong Wook Kim³, Jane Eunsoo Kim¹, Ye Young Kim¹, Yul Ji¹, Ji Seul Han¹, Sung Min Kim¹, Jee Hyung Sohn¹, Won Taek Lee¹, Sun Won Kim¹, Jeu Park¹, Jin Young Huh^1,4, Kyuri Jo⁵, Je-Yoel Cho³, Jiyoung Park² & Jae Bum Kim¹

¹Center for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul 08826, South Korea.

²Department of Biological Sciences, College of Information and Bioengineering, Ulsan National Institute of Science and Technology, Ulsan 44919, South Korea.

³Department of Biochemistry, BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.

⁴Department of Life Science, Sogang University, Seoul 04107, South Korea.

⁵Department of Computer Engineering, Chungbuk National University, Cheongju 28644, South Korea.

Corresponding author : Correspondence to Jae Bum Kim.

Abstract

In mammals, brown adipose tissue (BAT) and inguinal white adipose tissue (iWAT) execute sequential thermogenesis to maintain body temperature during cold stimuli. BAT rapidly generates heat through brown adipocyte activation, and further iWAT gradually stimulates beige fat cell differentiation upon prolonged cold challenges. However, fat depot-specific regulatory mechanisms for thermogenic activation of two fat depots are poorly understood. Here, we demonstrate that E3 ubiquitin ligase RNF20 orchestrates adipose thermogenesis with BAT- and iWAT-specific substrates. Upon cold stimuli, BAT RNF20 is rapidly downregulated, resulting in GABPα protein elevation by controlling protein stability, which stimulates thermogenic gene expression. Accordingly, BAT-specific Rnf20 suppression potentiates BAT thermogenic activity via GABPα upregulation. Moreover, upon prolonged cold stimuli, iWAT RNF20 is gradually upregulated to promote de novo beige adipogenesis. Mechanistically, iWAT RNF20 mediates NCoR1 protein degradation, rather than GABPα, to activate PPARγ. Together, current findings propose fat depot-specific regulatory mechanisms for temporal activation of adipose thermogenesis.

논문정보

형식Research article
게재일2024년 01월 (BRIC 등록일 2024-02-01)
연구진국내 연구진
분야 생명과학 > 분자생물학

댓글 작성 로그인

CV 열람하기

연락처 보기